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PMID: 2792077 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transient induction of IL-2 receptor in cultured T cell lines by HTLV-1 LTR-linked tax-1 gene.

The EMBO journal ·Vol. 8 ·No. 7 ·1989-07-00 ·Pages 1953-8

Doi T, Hatakeyama M, Itoh S, Taniguchi T

Abstract

Human lymphotropic virus, HTLV-1, encodes in its proviral genome a transcriptional activator protein, tax-1, that may be responsible for the development of virus-induced adult T cell leukemia (ATL), possibly through the aberrant activation of the genes for interleukin-2 (IL-2) and one of its receptor (IL-2R) components, the IL-2 receptor alpha-chain (IL-2R alpha). In the present study, an expression plasmid containing tax-1 cDNA under the control of HTLV-1 LTR was introduced into mouse and human CD4-positive T cell lines. Analysis of the established cell clones revealed a number of interesting features: (i) a limited fraction of the total cell population (less than 25% in each clone) was positive for IL-2R alpha; (ii) the IL-2R alpha expression was not permanent, as the IL-2R alpha positive and negative cells could convert either way. The experimental data suggest that the observed heterogeneity in IL-2R alpha expression in the transformants is due to a cell-cycle-regulated expression and function of tax-1. Furthermore, a proportion of the induced IL-2R in EL-4 was in high-affinity form, suggesting the association of the IL-2R alpha and the IL-2R beta chain (p70-75) components.

MeSH Terms
Animals Base Sequence Cell Cycle Cell Line DNA, Viral/genetics Flow Cytometry Genes, Viral Genetic Vectors Human T-lymphotropic virus 1/genetics Humans Molecular Sequence Data Oligonucleotide Probes Receptors, Interleukin-2/biosynthesis,genetics Repetitive Sequences, Nucleic Acid T-Lymphocytes/cytology,immunology Trans-Activators/genetics Transfection
Chemicals
DNA, Viral Oligonucleotide Probes Receptors, Interleukin-2 Trans-Activators
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Doi T
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Hatakeyama M
Itoh S
Taniguchi T
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-07-00
Pages
1953-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401056
Subset
IM
Corrections
ErratumIn
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External Links
PubMed source PMC Full Text View journal details
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