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PMID: 27931311 Published · ppublish English Journal Article

Cryopreservation of Cynomolgus Macaque (Macaca fascicularis) Sperm by Using a Commercial Egg-YolkFree Freezing Medium.

Journal of the American Association for Laboratory Animal Science : JAALAS ·Vol. 55 ·No. 6 ·2016-00-00 ·Pages 744-748

Yan Y, Ao L, Wang H, Duan Y, Chang S, Chen B, Zhi D, Li S, Niu Y, Ji W, Si W

Abstract

Conventional TRISegg yolk (TEY) freezing medium for the cryopreservation of NHP sperm has the risk of contamination due to widespread zoonotic diseases. This study was aimed at determining the optimal glycerol concentration, freezing rate, and holding time in liquid N2 vapor for the cryopreservation of cynomolgus macaque sperm by using a commercial egg-yolkfree freezing medium (SC medium) designed for human sperm cryopreservation. Sperm motility and acrosomal integrity after freezing were assessed. Sperm in SC medium (dilution ratio, 3:1) frozen at cooling rates of 67 and 183C/min in liquid N2 vapor showed higher post-thaw motility than did samples frozen at 435C/min. At the cooling rate of 183C/min and dilution in SC medium at a 3:1 ratio, post-thaw motility was higher after a holding time of 10 min than after 30 min (recommended by the manufacturer). In addition, post-thaw motility of sperm frozen in SC medium was higher with dilution ratios of 3:1, 4.5:1, and 6:1 compared with 9:1, 10.5:1, and 12:1, and the sample diluted 12:1 showed the lowest percentage of thawed sperm with intact acrosomes. Sperm showed higher post-thaw motility after freezing in TEY than in SC medium; acrosomal integrity did not differ between the 2 media. Our results indicated that cynomolgus macaque sperm can be cryopreserved successfully by using a commercial egg-yolkfree freezing medium, which provides an option for genetic preservation with decreased zoonotic risk in this important NHP species.

MeSH Terms
Animals Cryopreservation/methods,veterinary Cryoprotective Agents/chemistry Eggs Humans Macaca fascicularis Male Semen Preservation/methods,veterinary Sperm Motility Spermatozoa/cytology
Chemicals
Cryoprotective Agents
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Yan Yaping
Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
Ao Lei
Kunming SinoUK Angel Women's and Children's Hospital, Kunming, Yunnan, China.
Wang Hong
Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
Duan Yanchao
Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
Chang Shaohui
Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
Chen Bingbing
Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.
Zhi Dalong
Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
Li Sujuan
Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
Niu Yuyu
Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
Ji Weizhi
Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
Si Wei
Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China;, Email: [email protected].
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Article Info
Journal
Journal of the American Association for Laboratory Animal Science : JAALAS
Abbr.
J Am Assoc Lab Anim Sci
ISSN
1559-6109
Email
[email protected]
Published
2016-00-00
Pages
744-748
Language
English
Region
United States
NLM ID
101269489
PMCID
PMC5113874
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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