Somatic pairing of chromosome 1 centromeres in interphase nuclei of human cerebellum.

Human genetics ·Vol. 83 ·No. 3 ·1989-10-00 ·Pages 231-4

Arnoldus EP, Peters AC, Bots GT, Raap AK, van der Ploeg M

Abstract

Interphase nuclei isolated from paraffin-embedded tissue of four normal brains were hybridized with biotinated repetitive DNA probes specific for the (peri)centromeric regions of chromosomes 1 and 7. Hybridization results were visualized with a peroxidase-DAB system after which the number of specific signals per nucleus was counted using bright field microscopy. Using the probe specific for chromosome 7 (p7t1), both the cerebral and the cerebellar samples showed 2 spots in 82% and 83%, respectively, of the nuclei. In situ hybridization with the chromosome 1 probe (pUC1.77) showed two spots in 69% of the cerebral nuclei. In cerebellar samples, hybridization with pUC1.77 resulted in only one large spot per nucleus in 82% of the cells. The average spot size in nuclei with one signal was about 1.6 times as large as that in nuclei with two signals. These observations suggest that the single large spot in the cerebellar cells is not the result of monosomy of chromosome 1 but that it reflects somatic pairing of the two chromosome 1 centromeres. Based on the size and the fraction of nuclei with one large spot, the small granular neuron is the most likely candidate. The difference between cerebral and cerebellar samples indicates that this somatic pairing of chromosome 1 is a cell-type-dependent phenomenon.

MeSH Terms

Cell Nucleus/ultrastructure Centromere Cerebellum/cytology,ultrastructure Chromosomes, Human, Pair 1 DNA Probes Humans Immunohistochemistry Interphase Nucleic Acid Hybridization

Chemicals

DNA Probes

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Arnoldus E P

Department of Cytochemistry and Cytometry, Leiden University, The Netherlands.

Peters A C

Bots G T

Raap A K

van der Ploeg M

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Article Info

Journal

Human genetics

Abbr.

Hum Genet

ISSN

0340-6717

DOI

10.1007/BF00285162

Published

1989-10-00

Pages

231-4

Language

English

Region

Germany

NLM ID

7613873

Subset

External Links

PubMed source DOI: 10.1007/BF00285162 View journal details

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Somatic pairing of chromosome 1 centromeres in interphase nuclei of human cerebellum.

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