Home LiteratureArticle Details
PMID: 2798131 Published · ppublish English Comparative Study Journal Article

Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA.

Nucleic acids research ·Vol. 17 ·No. 19 ·1989-10-11 ·Pages 7843-53

Edwards U, Rogall T, Blöcker H, Emde M, Böttger EC

Abstract

Using a set of synthetic oligonucleotides homologous to broadly conserved sequences in-vitro amplification via the polymerase chain reaction followed by direct sequencing results in almost complete nucleotide determination of a gene coding for 16S ribosomal RNA. As a model system the nucleotide sequence of the 16S rRNA gene of M.kansasii was determined and found to be 98.7% homologous to that of M.bovis BCG. This is the first report on a contiguous sequence information of an entire amplified gene spanning 1.5 kb without any subcloning procedures.

MeSH Terms
Bacteria/genetics Base Sequence DNA-Directed DNA Polymerase Genes, Bacterial Molecular Sequence Data Mycobacterium/genetics Polymerase Chain Reaction RNA, Ribosomal/genetics RNA, Ribosomal, 16S/genetics Sequence Homology, Nucleic Acid
Chemicals
RNA, Ribosomal RNA, Ribosomal, 16S DNA-Directed DNA Polymerase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Edwards U
Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, FRG.
Rogall T
Blöcker H
Emde M
Böttger E C
References (23)
23 references, click to expand
  1. Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells.
    J Bacteriol. 1988 Feb;170(2):720-6 PMID: 2448289
  2. Polymerase chain reaction reveals cloning artefacts.
    Nature. 1988 Aug 4;334(6181):387-8 PMID: 2841606
  3. Errors in the polymerase chain reaction.
    Nucleic Acids Res. 1988 Nov 11;16(21):10393 PMID: 3194225
  4. Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.
    Proc Natl Acad Sci U S A. 1988 Oct;85(20):7652-6 PMID: 3174659
  5. Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells.
    Science. 1989 Mar 10;243(4896):1360-3 PMID: 2466341
  6. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses.
    Proc Natl Acad Sci U S A. 1985 Oct;82(20):6955-9 PMID: 2413450
  7. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
    Science. 1985 Dec 20;230(4732):1350-4 PMID: 2999980
  8. Species-specific oligonucleotide probes for rRNA of Clostridium difficile and related species.
    J Clin Microbiol. 1988 Dec;26(12):2484-8 PMID: 3230127
  9. Bacterial evolution.
    Microbiol Rev. 1987 Jun;51(2):221-71 PMID: 2439888
  10. Complete nucleotide sequence of the 16S rRNA gene of Mycobacterium bovis BCG.
    J Bacteriol. 1988 Jun;170(6):2886-9 PMID: 3286624
  11. DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.
    Proc Natl Acad Sci U S A. 1988 Dec;85(24):9436-40 PMID: 3200828
  12. On the evolutionary descent of organisms and organelles: a global phylogeny based on a highly conserved structural core in small subunit ribosomal RNA.
    Nucleic Acids Res. 1984 Jul 25;12(14):5837-52 PMID: 6462918
  13. Direct sequencing of polymerase chain reaction amplified DNA fragments through the incorporation of deoxynucleoside alpha-thiotriphosphates.
    Nucleic Acids Res. 1988 Nov 11;16(21):9947-59 PMID: 3194230
  14. HLA-DQ beta gene contributes to susceptibility and resistance to insulin-dependent diabetes mellitus.
    Nature. 1987 Oct 15-21;329(6140):599-604 PMID: 3309680
  15. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
    Science. 1988 Jan 29;239(4839):487-91 PMID: 2448875
  16. Compilation of small ribosomal subunit RNA sequences.
    Nucleic Acids Res. 1988;16 Suppl:r87-173 PMID: 2453029
  17. Synthetic oligonucleotide probes complementary to rRNA for group- and species-specific detection of mycoplasmas.
    Isr J Med Sci. 1987 Jun;23(6):742-6 PMID: 2889700
  18. Length mutations in human mitochondrial DNA: direct sequencing of enzymatically amplified DNA.
    Nucleic Acids Res. 1987 Jan 26;15(2):529-42 PMID: 2881260
  19. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
    Methods Enzymol. 1987;155:335-50 PMID: 3431465
  20. A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions.
    Nucleic Acids Res. 1988 Aug 11;16(15):7351-67 PMID: 3045756
  21. Rapid ribosomal RNA sequencing and the phylogenetic analysis of protists.
    Parasitol Today. 1989 Apr;5(4):102-5 PMID: 15463187
  22. Characterization of beta-thalassaemia mutations using direct genomic sequencing of amplified single copy DNA.
    Nature. 1987 Nov 26-Dec 2;330(6146):384-6 PMID: 3683554
  23. Direct cloning and sequence analysis of enzymatically amplified genomic sequences.
    Science. 1986 Sep 5;233(4768):1076-8 PMID: 3461561
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.19.7843
Published
1989-10-11
Pages
7843-53
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334891
Subset
IM
Databases
GENBANK
X15916
External Links
PubMed source DOI: 10.1093/nar/17.19.7843 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2