Kinetics of inhibition by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole on calf thymus casein kinase II.
The adenosine analogue 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) is a specific inhibitor for RNA polymerase II transcription in vivo and in vitro [Tamm + Sehgal (1978) Adv. Virus Res. 22, 187-258; Zandomeni & Weinmann (1984) J. Biol. Chem. 259, 14804-14811]. The effect on RNA polymerase II-specific transcription seems to be mediated by its inhibition of nuclear casein kinase II [Zandomeni, Carrera-Zandomeni, Shugar & Weinmann (1986) J. Biol. Chem. 261, 3414-3419]. Inhibition studies indicated that DRB acted as a mixed-type inhibitor with respect to casein and as a competitive inhibitor with respect to the nucleotide phosphate donor substrates. The DRB inhibition constant is 7 microM for the calf thymus casein kinase II, with regard to both ATP and GTP.
25 references, click to expand
-
Mechanism of action of dichloro-beta-D-ribofuranosylbenzimidazole: effect on in vitro transcription.Proc Natl Acad Sci U S A. 1982 May;79(10):3167-70 PMID: 6954467
-
A heparin-sensitive nuclear protein kinase. Purification, properties, and increased activity in rat hepatoma relative to liver.J Biol Chem. 1981 Jul 25;256(14):7468-77 PMID: 7251604
-
Purification and characterization of a type II casein kinase from Drosophila melanogaster.J Biol Chem. 1983 Mar 10;258(5):3258-65 PMID: 6298230
-
Rapidly phosphorylated histone-like proteins are modulated in the course of transcription block by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole.Eur J Cell Biol. 1983 Sep;31(2):354-9 PMID: 6641743
-
Dinucleotide primers facilitate convenient identification of the mouse ribosomal DNA transcription initiation site. A general method for analysis of transcription by RNA polymerases I and III.J Biol Chem. 1983 Nov 25;258(22):13919-28 PMID: 6315712
-
Similarities in structure and function of calf thymus and Drosophila casein kinase II.J Biol Chem. 1984 Jul 25;259(14):9001-6 PMID: 6589223
-
Inhibitory effect of 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole on a protein kinase.J Biol Chem. 1984 Dec 10;259(23):14804-11 PMID: 6501318
-
Casein kinase type II is involved in the inhibition by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole of specific RNA polymerase II transcription.J Biol Chem. 1986 Mar 5;261(7):3414-9 PMID: 3456346
-
Selective repression of RNA polymerase II by microinjected phosvitin.Chromosoma. 1986;94(5):329-36 PMID: 3470171
-
Calf brain phosvitin kinase. II. Purification and characterization of three different fractions of phosvitin kinase.Biochim Biophys Acta. 1973 Jan 12;293(1):140-9 PMID: 4734372
-
A tentative initiation inhibitor of chromosomal heterogeneous RNA synthesis.J Mol Biol. 1974 Mar 25;84(1):173-83 PMID: 4857470
-
Stimulation of ascites tumor RNA polymerase II by protein kinase.Biochemistry. 1976 May 4;15(9):1821-9 PMID: 178356
-
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
-
Effects of polyamines and polyanions on a cyclic nucleotide-independent and a cyclic AMP-dependent protein kinase.Biochim Biophys Acta. 1977 Jul 21;498(1):294-305 PMID: 195631
-
Purification of rat liver nuclear protein kinase NII.J Biol Chem. 1977 Oct 10;252(19):6660-5 PMID: 893433
-
Halobenzimidazole ribosides and RNA synthesis of cells and viruses.Adv Virus Res. 1978;22:187-258 PMID: 345776
-
Regulation of DNA replication in S phase nuclei by ATP and ADP pools.Proc Natl Acad Sci U S A. 1979 Apr;76(4):1643-7 PMID: 287004
-
Purification and characterization of a casein kinase from human erythrocyte cytosol.Biochem Biophys Res Commun. 1979 Jul 12;89(1):7-16 PMID: 475816
-
Studies of subunit structure of phosvitin kinase FII from bovine brain.FEBS Lett. 1979 Dec 1;108(1):200-4 PMID: 520546
-
Two naturally occurring inhibitors of nuclear protein kinase.J Biol Chem. 1980 May 25;255(10):4589-94 PMID: 7372596
-
Selective inhibition of a cyclic nucleotide-independent protein kinase (G-type casein kinase) by naturally occurring glycosaminoglycans.FEBS Lett. 1980 Nov 17;121(1):139-42 PMID: 6161840
-
Compartmentalized ATP pools produced from adenosine are nuclear pools.J Cell Physiol. 1980 Nov;105(2):267-74 PMID: 6450772
-
Purification and properties of calf thymus casein kinases I and II.J Biol Chem. 1981 Apr 10;256(7):3319-25 PMID: 6937462
-
Cyclic nucleotide independent casein kinase (G type) in bovine adrenal cortex: purification and properties of two molecular forms.Biochim Biophys Acta. 1981 Apr 14;658(2):191-201 PMID: 6941814
-
Catalytic and molecular properties of a highly purified G type casein kinase from bovine lung tissue.Biochim Biophys Acta. 1983 Feb 28;743(1):1-12 PMID: 6572070
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
