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PMID: 2803273 Published · ppublish English Journal Article

Identification of a novel autophosphorylation site (P4) on the epidermal growth factor receptor.

The Biochemical journal ·Vol. 262 ·No. 2 ·1989-09-01 ·Pages 659-63

Hsuan JJ, Totty N, Waterfield MD

Abstract

Three major autophosphorylation sites are located near the C-terminus of the epidermal growth factor receptor, but a fourth site is repeatedly detected. We report here the purification and sequencing of a tryptic peptide containing this site, Tyr-1086. Furthermore, we demonstrate that additional phosphopeptides are observed following both partial digestion and overdigestion. Finally, we show that Tyr-1086 can be phosphorylated in intact cells.

MeSH Terms
Amino Acid Sequence ErbB Receptors/isolation & purification Humans Molecular Sequence Data Peptide Mapping Phosphorylation
Chemicals
ErbB Receptors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hsuan J J
Structural Biology Group, Ludwig Institute for Cancer Research, London, U.K.
Totty N
Waterfield M D
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33 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2620659
Published
1989-09-01
Pages
659-63
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1133318
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2620659 PMC Full Text View journal details
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