In vitro activity of vancomycin against enterococci.

The in vitro activity of vancomycin against 40 clinical isolates of enterococci was determined by a macro-tube dilution method and by quantitative killing curve procedures employing the standard medium of our department, i.e. a filtered ox broth. An attempt to remove the influence of technical factors on the MBC determination was made by using an inoculum in the early logarithmic growth phase and ensuring the exposure of all the organisms to the antibiotic. Vancomycin showed a good inhibitory activity for the enterococci tested (MIC90 of 1.6 micrograms/ml, 3.1 micrograms/ml and 1.6 micrograms/ml for S. faecalis, S. faecium and S. durans, respectively), but no bactericidal effect could be demonstrated as measured by the MBCs (greater than 100 micrograms/ml) and killing curve procedures.