Primary structure of two neuropeptide hormones with adipokinetic and hypotrehalosemic activity isolated from the corpora cardiaca of horse flies (Diptera).

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 20 ·1989-10-00 ·Pages 8161-4

Jaffe H, Raina AK, Riley CT, Fraser BA, Nachman RJ, Vogel VW, Zhang YS, Hayes DK

Abstract

The primary structures of two neuropeptides, Tabanus atratus adipokinetic hormone (Taa-AKH) and Tabanus atratus hypotrehalosemic hormone (Taa-HoTH), from the corpora cardiaca of horse flies (Diptera: Tabanidae) have been determined. Amino acid sequences of Taa-AKH (less than Glu-Leu-Thr-Phe-Thr-Pro-Gly-Trp-NH2) and Taa-HoTH (less than Glu-Leu-Thr-Phe-Thr-Pro-Gly-Trp-Gly-Tyr-NH2) (where less than Glu = pyroglutamic acid) were determined by automated gas-phase Edman degradation of the peptides deblocked by pyroglutamate aminopeptidase and by fast atom bombardment mass spectrometry. The hormones were synthesized, and the natural and synthetic peptides exhibited identical chromatographic, spectroscopic, and biological properties. When assayed in adult face fly males, Taa-AKH and Taa-HoTH demonstrated hyperlipemic activity, in addition, Taa-HoTH also demonstrated a significant hypotrehalosemic activity.

MeSH Terms

Amino Acid Sequence Animals Diptera/drug effects,physiology Insect Hormones/chemical synthesis,pharmacology Male Mass Spectrometry Molecular Sequence Data Nervous System Physiological Phenomena Neuropeptides Oligopeptides/chemical synthesis,pharmacology Pyrrolidonecarboxylic Acid/analogs & derivatives

Chemicals

Insect Hormones Neuropeptides Oligopeptides hypotrehalosemic hormone, Tabanus atratus adipokinetic hormone, Tabanus atratus Pyrrolidonecarboxylic Acid

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Jaffe H

Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705.

Raina A K

Riley C T

Fraser B A

Nachman R J

Vogel V W

Zhang Y S

Hayes D K

References (7)

7 references, click to expand

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Insect hypertrehalosemic hormone: isolation and primary structure from Blaberus discoidalis cockroaches.
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Isolation of two neuropeptides in the AKH/RPCH-family from horseflies (Diptera).
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Isolation and primary structure of a peptide from the corpora cardiaca of Heliothis zea with adipokinetic activity.
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.86.20.8161

Published

1989-10-00

Pages

8161-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC298235

Subset

External Links

PubMed source DOI: 10.1073/pnas.86.20.8161 PMC Full Text View journal details

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Primary structure of two neuropeptide hormones with adipokinetic and hypotrehalosemic activity isolated from the corpora cardiaca of horse flies (Diptera).

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