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PMID: 2813405 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A nuclear protein associated with human cancer cells binds preferentially to a human repetitive DNA sequence.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 21 ·1989-11-00 ·Pages 8472-6

Law ML, Gao JZ, Puck TT

Abstract

A protein (Rp66) of 66 kDa was shown by DNA-binding protein blot assay to bind to a human repetitive DNA sequence (low-repeat sequence; LRS) in each of 10 transformed human cell lines examined. This protein-DNA interaction was not observed in 11 normal human cell cultures or in the Chinese hamster cell line CHO-K1. Gel retardation assay confirmed the specificity of the protein-DNA binding between Rp66 and LRS. In a histiocytic lymphoma human cell line, U937, that can be induced to differentiate in the presence of phorbol ester, this binding disappeared after cell differentiation. These together with other results cited suggest a regulatory role for these repetitive sequences in the human genome, with particular application to cancer.

MeSH Terms
Animals Cell Line Cricetinae DNA-Binding Proteins/isolation & purification,metabolism Electrophoresis, Polyacrylamide Gel Humans Molecular Weight Neoplasm Proteins/isolation & purification,metabolism Nuclear Proteins/isolation & purification,metabolism Repetitive Sequences, Nucleic Acid Tetradecanoylphorbol Acetate/pharmacology
Chemicals
DNA-Binding Proteins Neoplasm Proteins Nuclear Proteins Rp66 protein, human Tetradecanoylphorbol Acetate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Law M L
Sam and Freda Davis Laboratory, Eleanor Roosevelt Institute for Cancer Research, Denver, CO 80206.
Gao J Z
Puck T T
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.21.8472
Published
1989-11-00
Pages
8472-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298304
Subset
IM
Grants
NIGMS NIH HHS · GM33903 · United States
NICHD NIH HHS · HD02080 · United States
NICHD NIH HHS · HD17449 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.21.8472 PMC Full Text View journal details
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