Home LiteratureArticle Details
PMID: 281714 Published · ppublish English Journal Article

Molecular nature of the complement lesion.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 11 ·1978-11-00 ·Pages 5655-9

Bhakdi S, Tranum-Jensen J

Abstract

The principle molecular event leading to membrane perturbation by complement is the assembly of the terminal five serum complement components (C5b-C9) into a macromolecular C5b-9 complex on the target membrane [Müller-Eberhard, H.-J. (1975) Ann. Rev. Biochem. 44, 697--723]. The present communication reports on the ability of purified C5b-9 complexes isolated from target membranes to become reincorporated into artificial lipid vesicles. The data indicate that the complex is a vertically oriented, hollow, cylindrical macromolecule possessing lipid-binding regions that enable one terminus to penetrate into the lipid bilayer. A transmembrane pore appears to be created at the attachment site of the C5b-9 complex.

MeSH Terms
Animals Complement System Proteins Erythrocyte Membrane/ultrastructure Humans Immunoelectrophoresis Membrane Lipids/blood Membranes, Artificial Microscopy, Electron Sheep
Chemicals
Membrane Lipids Membranes, Artificial Complement System Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bhakdi S
Tranum-Jensen J
References (35)
35 references, click to expand
  1. LESIONS IN ERYTHROCYTE MEMBRANES CAUSED BY IMMUNE HAEMOLYSIS.
    Nature. 1964 Apr 18;202:251-2 PMID: 14167777
  2. Complement-mediated lysis of liposomes produced by the reactive lysis procedure.
    Immunology. 1970 Dec;19(6):983-6 PMID: 5530596
  3. The structural events associated with the attachment of complement components to cell membranes in reactive lysis.
    Immunology. 1978 Aug;35(2):205-12 PMID: 750370
  4. On the mechanism of membrane damage by C: exposure of hydrophobic sites on activated C proteins.
    J Immunol. 1977 Oct;119(4):1358-64 PMID: 561127
  5. Release of phospholipids from liposomal model membrane damaged by antibody and complement.
    J Immunol. 1977 Jul;119(1):73-6 PMID: 559702
  6. Characterization of membrane proteins in detergent solutions.
    Biochim Biophys Acta. 1976 Oct 26;457(2):133-70 PMID: 135582
  7. On the mechanism of cell membrane damage by complement: evidence on insertion of polypeptide chains from C8 and C9 into the lipid bilayer of erythrocytes.
    J Immunol. 1977 Jul;119(1):1-8 PMID: 559700
  8. Complement lysis: the ultrastructure and orientation of the C5b-9 complex on target sheep erythrocyte membranes.
    Scand J Immunol. 1978;7(1):45-6 PMID: 635472
  9. Reconstitution of Semliki forest virus membrane.
    J Cell Biol. 1977 Dec;75(3):866-80 PMID: 925085
  10. Complement-induced ultrastructural membrane lesions: requirement for terminal components.
    J Immunol. 1976 Nov;117(5 Pt.2):1883-9 PMID: 993584
  11. The binding of detergents to lipophilic and hydrophilic proteins.
    J Biol Chem. 1972 Jun 10;247(11):3656-61 PMID: 4113126
  12. Mechanism of cytolysis by complement.
    Proc Natl Acad Sci U S A. 1972 Oct;69(10):2954-8 PMID: 4117012
  13. Reactive lysis: the complement-mediated lysis of unsensitized cells. II. The characterization of activated reactor as C56 and the participation of C8 and C9.
    J Exp Med. 1970 Apr 1;131(4):643-57 PMID: 4193935
  14. Apparent dependence of interactions between cytochrome b5 and cytochrome b5 reductase upon translational diffusion in dimyristoyl lecithin liposomes.
    Proc Natl Acad Sci U S A. 1975 Jul;72(7):2658-61 PMID: 1058480
  15. The fluid mosaic model of the structure of cell membranes.
    Science. 1972 Feb 18;175(4023):720-31 PMID: 4333397
  16. Studies on the terminal stages of complement lysis.
    Immunology. 1973 Jan;24(1):135-45 PMID: 4346598
  17. Dissociation and reconstitution of functional sarcoplasmic reticulum vesicles.
    J Biol Chem. 1974 Jan 10;249(1):302-9 PMID: 4272159
  18. Reconstitution of a calcium pump using defined membrane components.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):622-6 PMID: 4274560
  19. Complement-induced changes in the core structure of sheep erythrocyte membranes: a study by freeze-etch electron microscopy.
    Biochim Biophys Acta. 1974 Aug 9;356(3):300-8 PMID: 4210701
  20. Antibody-complement interaction with lipid model membranes.
    Biochim Biophys Acta. 1972 Feb 14;265(1):1-23 PMID: 4554562
  21. Mode of action of human C9: adsorption of multiple C9 molecules to cell-bound C8.
    J Immunol. 1974 Aug;113(2):479-88 PMID: 4858623
  22. Molecular analysis of the membrane attack mechanism of complement.
    J Exp Med. 1972 Mar 1;135(3):549-66 PMID: 5058233
  23. Lesions due to complement in lipid membranes.
    Nature. 1971 Oct 29;233(5322):620-3 PMID: 4939804
  24. The lesions in cell membranes caused by complement.
    Adv Immunol. 1969;11:75-115 PMID: 4908393
  25. Immunochemical analyses of membrane-bound complement. Detection of the terminal complement complex and its similarity to "intrinsic" erythrocyte membrane proteins.
    Biochim Biophys Acta. 1975 Sep 16;406(1):21-35 PMID: 809065
  26. Mechanism of complement-induced cell lysis. Demonstration of a three-step mechanism of EAC1-8 cell lysis by C9 and of a non-osmotic swelling of erythrocytes.
    J Immunol. 1975 Oct;115(4):1028-33 PMID: 809505
  27. Solubilization of membranes by detergents.
    Biochim Biophys Acta. 1975 Mar 25;415(1):29-79 PMID: 1091302
  28. Complement.
    Annu Rev Biochem. 1975;44:697-724 PMID: 1094920
  29. Mammalian plasma membranes.
    Nature. 1975 Nov 6;258(5530):43-9 PMID: 1102991
  30. Phospholipid metabolism of stimulated lymphocytes. Composition of phospholipid fatty acids.
    Biochim Biophys Acta. 1975 Sep 19;398(3):364-76 PMID: 1100108
  31. Increased ion permeability of planar lipid bilayer membranes after treatment with the C5b-9 cytolytic attack mechanism of complement.
    Proc Natl Acad Sci U S A. 1976 Aug;73(8):2852-6 PMID: 1066698
  32. On the mechanism of cytolysis by complement: evidence on insertion of C5b and C7 subunits of the C5b,6,7 complex into phospholipid bilayers of erythrocyte membranes.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):5076-80 PMID: 1061092
  33. The indiction by complement of a change in KSCN-dissociable red cell membrane lipids.
    J Immunol. 1976 Apr;116(4):1163-9 PMID: 1254965
  34. Isolation of the terminal complement complex from target sheep erythrocyte membranes.
    Biochim Biophys Acta. 1976 Feb 6;419(3):445-57 PMID: 1247570
  35. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes.
    Arch Biochem Biophys. 1963 Jan;100:119-30 PMID: 14028302
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.11.5655
Published
1978-11-00
Pages
5655-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393026
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.11.5655 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2