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PMID: 2819870 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In situ detection of enteroviral genomes in myocardial cells by nucleic acid hybridization: an approach to the diagnosis of viral heart disease.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 17 ·1987-09-00 ·Pages 6272-6

Kandolf R, Ameis D, Kirschner P, Canu A, Hofschneider PH

Abstract

We have developed an in situ hybridization assay capable of detecting enteroviral RNA in myocardial cells, using molecularly cloned coxsackievirus B3 cDNA as a diagnostic probe. Because of the high degree of nucleic acid sequence homology among the numerous enteroviral serotypes, including the group A and B coxsackieviruses and the echoviruses, detection of these various agents commonly implicated in human viral heart disease is possible in a single hybridization assay. We demonstrate the considerable potential of this method for an unequivocal diagnosis of enteroviral heart disease as well as for pathogenicity studies. Using athymic mice persistently infected with coxsackievirus B3 as a model system, we show that the myocardium is affected in a disseminated, multifocal manner.

MeSH Terms
Animals DNA/genetics Enterovirus/genetics,isolation & purification Enterovirus B, Human/genetics Enterovirus Infections/diagnosis Genes, Viral Heart/microbiology Mice Myocarditis/diagnosis Nucleic Acid Hybridization RNA, Viral/genetics
Chemicals
RNA, Viral DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kandolf R
Ameis D
Kirschner P
Canu A
Hofschneider P H
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.17.6272
Published
1987-09-00
Pages
6272-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299053
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.17.6272 PMC Full Text View journal details
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