Home LiteratureArticle Details
PMID: 2820945 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Bacteriophage K20 requires both the OmpF porin and lipopolysaccharide for receptor function.

Journal of bacteriology ·Vol. 169 ·No. 10 ·1987-10-00 ·Pages 4830-3

Silverman JA, Benson SA

Abstract

Mutations which prevent absorption of the bacteriophage K20 to Escherichia coli K-12 were selected by using an altered OmpF protein which confers the ability to grow on maltodextrin in the absence of the LamB maltoporin. The mutations map in the rfa gene cluster and alter the structure of the lipopolysaccharide core.

MeSH Terms
Alleles Anti-Bacterial Agents/pharmacology Bacterial Outer Membrane Proteins/metabolism Cell Membrane Permeability Coliphages/metabolism Escherichia coli/drug effects,genetics,growth & development Genes, Bacterial Genetic Linkage Lipopolysaccharides/metabolism Mutation Phenotype Polysaccharides/metabolism Porins Receptors, Virus/metabolism Transduction, Genetic
Chemicals
Anti-Bacterial Agents Bacterial Outer Membrane Proteins Lipopolysaccharides Polysaccharides Porins Receptors, Virus maltoporins maltodextrin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Silverman J A
Department of Molecular Biology, Princeton University, New Jersey 08544.
Benson S A
References (18)
18 references, click to expand
  1. Bacteriophage resistance in Escherichia coli K-12: general pattern of resistance.
    J Bacteriol. 1975 Mar;121(3):983-93 PMID: 1090611
  2. Novel regulatory mutants of the phosphate regulon in Escherichia coli K-12.
    J Mol Biol. 1986 Sep 5;191(1):39-58 PMID: 3540312
  3. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  4. Genetic study of a membrane protein: DNA sequence alterations due to 17 lamB point mutations affecting adsorption of phage lambda.
    EMBO J. 1983;2(1):77-80 PMID: 11894913
  5. Fractions of lipopolysaccharide from Escherichia coli O111:B4 prepared by two extraction procedures.
    J Biol Chem. 1975 Apr 25;250(8):2911-19 PMID: 804483
  6. Major proteins of the Escherichia coli outer cell envelope membrane. Interaction of protein II with lipopolysaccharide.
    Eur J Biochem. 1978 Jan 2;82(1):211-7 PMID: 340230
  7. Role of lipopolysaccharide and outer membrane protein of Escherichia coli K-12 in the receptor activity for bacteriophage T4.
    J Bacteriol. 1978 Nov;136(2):693-9 PMID: 361717
  8. Location of a phage binding region on an outer membrane protein.
    FEBS Lett. 1980 Nov 17;121(1):127-9 PMID: 7007075
  9. Regulation of the OmpA outer membrane protein of Escherichia coli.
    J Bacteriol. 1981 Sep;147(3):972-85 PMID: 7024253
  10. Mutations that alter the transport function of the LamB protein in Escherichia coli.
    J Bacteriol. 1982 Jul;151(1):15-21 PMID: 6211431
  11. Topology of phage lambda receptor protein. Mapping targets of proteolytic cleavage in relation to binding sites for phage or monoclonal antibodies.
    J Biol Chem. 1984 Jun 25;259(12):7570-6 PMID: 6234303
  12. Escherichia coli K-12 outer membrane protein (OmpA) as a bacteriophage receptor: analysis of mutant genes expressing altered proteins.
    J Bacteriol. 1984 Aug;159(2):570-8 PMID: 6086577
  13. Host range mutants of bacteriophage Ox2 can use two different outer membrane proteins of Escherichia coli K-12 as receptors.
    J Bacteriol. 1984 Aug;159(2):579-82 PMID: 6378883
  14. Isolation and characterization of outer membrane permeability mutants in Escherichia coli K-12.
    J Bacteriol. 1985 Jan;161(1):361-7 PMID: 2981807
  15. New cysE-pyrE-linked rfa mutation in Escherichia coli K-12 that results in a heptoseless lipopolysaccharide.
    J Bacteriol. 1985 Mar;161(3):1209-14 PMID: 3882666
  16. Localization of functional domains in E. coli K-12 outer membrane porins.
    EMBO J. 1985 Jun;4(6):1583-7 PMID: 2411544
  17. Porin from bacterial and mitochondrial outer membranes.
    CRC Crit Rev Biochem. 1985;19(2):145-90 PMID: 2415299
  18. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels.
    J Bacteriol. 1983 Apr;154(1):269-77 PMID: 6187729
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1987-10-00
Pages
4830-3
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213862
Subset
IM
Grants
NIGMS NIH HHS · GM34821 · United States
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]