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PMID: 2821075 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Induction of the skeletal alpha-actin gene in alpha 1-adrenoceptor-mediated hypertrophy of rat cardiac myocytes.

The Journal of clinical investigation ·Vol. 80 ·No. 4 ·1987-10-00 ·Pages 1194-9

Bishopric NH, Simpson PC, Ordahl CP

Abstract

Myocardial hypertrophy in vivo is associated with reexpression of contractile protein isogenes characteristic of fetal and neonatal development. The molecular signals for hypertrophy and isogene switching are unknown. We studied alpha (sarcomeric)-actin messenger RNA (mRNA) expression in cultured cardiac myocytes from the neonatal rat. In the cultured cells, as in the adult heart in vivo, expression of cardiac alpha-actin (cACT) predominated over that of skeletal alpha-actin (sACT) mRNA, the fetal/neonatal isoform. alpha 1-Adrenergic receptor stimulation induced hypertrophy of these cells, increasing total RNA and cytoskeletal actin mRNA by 1.8-fold over control, and total alpha-actin mRNA by 4.3 fold. This disproportionate increase in total alpha-actin mRNA was produced by a preferential induction of sACT mRNA, which increased by 10.6-fold over control versus only 2.6-fold for cACT mRNA. The alpha 1-adrenoceptor is the first identified molecular mediator of early developmental isogene reexpression in cardiac myocyte hypertrophy.

MeSH Terms
Actins/genetics Animals Cardiomegaly/metabolism Cells, Cultured Dose-Response Relationship, Drug Isoproterenol/pharmacology Myocardial Contraction Norepinephrine/pharmacology Prazosin/analogs & derivatives,pharmacology Propranolol/pharmacology RNA, Messenger/metabolism Rats Receptors, Adrenergic, alpha/metabolism
Chemicals
Actins RNA, Messenger Receptors, Adrenergic, alpha Terazosin Propranolol Isoproterenol Norepinephrine Prazosin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bishopric N H
Department of Anatomy, University of California, San Francisco 94143.
Simpson P C
Ordahl C P
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI113179
Published
1987-10-00
Pages
1194-9
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC442365
Subset
IM
Grants
NHLBI NIH HHS · R01 HL031113 · United States
NHLBI NIH HHS · HL-07175 · United States
NHLBI NIH HHS · HL31113 · United States
NHLBI NIH HHS · HL35561 · United States
External Links
PubMed source DOI: 10.1172/JCI113179 PMC Full Text View journal details
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