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PMID: 2823064 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of major outer membrane porin proteins of Escherichia coli K 12 by pH.

Molecular & general genetics : MGG ·Vol. 208 ·No. 3 ·1987-07-00 ·Pages 511-7

Heyde M, Portalier R

Abstract

Electrophoretic analysis of total membrane proteins of Escherichia coli cells grown at neutral or acid pH showed that ompF, ompC and lamB porin gene expression was regulated by changes in extracellular pH. Growth at acid pH was correlated with a decrease in outer membrane proteins OmpF and LamB and an increase in protein OmpC. pH-induced effects were confirmed and quantitatively estimated by using strains carrying ompF-lacZ, ompC-lacZ and lamB-lacZ fusions. Our studies showed that the pH-dependent regulation acted at a transcriptional level on ompF and ompC gene expression and also at a post-transcriptional level on ompF gene expression. Similar studies carried out with envZ strains showed that the pH-controlled transducing signal was mediated via the EnvZ protein, although other pH-dependent pathways were also involved.

MeSH Terms
Bacterial Outer Membrane Proteins/genetics Escherichia coli/genetics,growth & development Gene Expression Regulation Genes Genes, Bacterial Genes, Regulator Genotype Hydrogen-Ion Concentration Kinetics Porins beta-Galactosidase/genetics,metabolism
Chemicals
Bacterial Outer Membrane Proteins Porins beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Heyde M
Atelier de Biotechnologie du Centre National de la Recherche Scientifique, Université Claude Bernard Lyon I, Villeurbanne, France.
Portalier R
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00328148
Published
1987-07-00
Pages
511-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00328148
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