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PMID: 2823136 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A cDNA clone expressing a rhoptry protein of Plasmodium falciparum.

Molecular and biochemical parasitology ·Vol. 25 ·No. 1 ·1987-08-00 ·Pages 73-81

Coppel RL, Bianco AE, Culvenor JG, Crewther PE, Brown GV, Anders RF, Kemp DJ

Abstract

Antibodies from immune humans were used to select a cDNA clone expressing an asexual blood stage antigen of Plasmodium falciparum. The expressed fused polypeptide was used as an affinity reagent to purify human antibodies specific for the corresponding parasite antigen. Western blotting and immunoelectronmicroscopy demonstrated that the antigen was a 105 kDa protein located in the rhoptries of merozoites. The cDNA encodes the carboxy terminus of the rhoptry antigen, a sequence rich both in charged and hydroxy amino acids.

MeSH Terms
Amino Acid Sequence Animals Antigens, Protozoan/genetics Base Sequence Chromatography, Affinity Cloning, Molecular DNA/genetics DNA Restriction Enzymes Deoxyribonuclease EcoRI Deoxyribonuclease HindIII Fluorescent Antibody Technique Genes Humans Immunoassay Microscopy, Electron Molecular Sequence Data Nucleic Acid Hybridization Plasmodium falciparum/genetics,immunology,ultrastructure
Chemicals
Antigens, Protozoan DNA DNA Restriction Enzymes Deoxyribonuclease EcoRI Deoxyribonuclease HindIII
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Coppel R L
Walter and Eliza Hall Institute of Medical Research, Victoria, Australia.
Bianco A E
Culvenor J G
Crewther P E
Brown G V
Anders R F
Kemp D J
Article Info
Journal
Molecular and biochemical parasitology
Abbr.
Mol Biochem Parasitol
ISSN
0166-6851
DOI
10.1016/0166-6851(87)90020-x
Published
1987-08-00
Pages
73-81
Language
English
Region
Netherlands
NLM ID
8006324
Subset
IM
Databases
GENBANK
M17530
External Links
PubMed source DOI: 10.1016/0166-6851(87)90020-x View journal details
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