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PMID: 2823261 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 21 ·1987-11-00 ·Pages 7413-7

Felgner PL, Gadek TR, Holm M, Roman R, Chan HW, Wenz M, Northrop JP, Ringold GM, Danielsen M

Abstract

A DNA-transfection protocol has been developed that makes use of a synthetic cationic lipid, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA). Small unilamellar liposomes containing DOTMA interact spontaneously with DNA to form lipid-DNA complexes with 100% entrapment of the DNA, DOTMA facilitates fusion of the complex with the plasma membrane of tissue culture cells, resulting in both uptake and expression of the DNA. The technique is simple, highly reproducible, and effective for both transient and stable expression of transfected DNA. Depending upon the cell line, lipofection is from 5- to greater than 100-fold more effective than either the calcium phosphate or the DEAE-dextran transfection technique.

MeSH Terms

Animals Calcium Phosphates Cell Line DEAE-Dextran Genetic Engineering/methods Indicators and Reagents L Cells/cytology Liposomes Quaternary Ammonium Compounds/chemical synthesis Simian virus 40/genetics Transfection

Chemicals

Calcium Phosphates Indicators and Reagents Liposomes Quaternary Ammonium Compounds N-(1-(2,3-dioleyloxy)propyl)-N,N,N-trimethylammonium DEAE-Dextran

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Felgner P L

Institute of Bio-Organic Chemistry, Syntex Research, Palo Alto, CA 94303.

Gadek T R

Holm M

Roman R

Chan H W

Wenz M

Northrop J P

Ringold G M

Danielsen M

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.84.21.7413

Published

1987-11-00

Pages

7413-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC299306

Subset

Grants

NIGMS NIH HHS · GM25821 · United States

External Links

PubMed source DOI: 10.1073/pnas.84.21.7413 PMC Full Text View journal details

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