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PMID: 2824992 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 7 ·No. 10 ·1987-10-00 ·Pages 3629-36

Nikawa J, Sass P, Wigler M

Abstract

Saccharomyces cerevisiae contains two genes which encode cyclic AMP (cAMP) phosphodiesterase. We previously isolated and characterized PDE2, which encodes a high-affinity cAMP phosphodiesterase. We have now isolated the PDE1 gene of S. cerevisiae, which encodes a low-affinity cAMP phosphodiesterase. These two genes represent highly divergent branches in the evolution of phosphodiesterases. High-copy-number plasmids containing either PDE1 or PDE2 can reverse the growth arrest defects of yeast cells carrying the RAS2(Val-19) mutation. PDE1 and PDE2 appear to account for the aggregate cAMP phosphodiesterase activity of S. cerevisiae. Disruption of both PDE genes results in a phenotype which resembles that induced by the RAS2(Val-19) mutation. pde1- pde2- ras1- ras2- cells are viable.

MeSH Terms
3',5'-Cyclic-AMP Phosphodiesterases/genetics Amino Acid Sequence Base Sequence Cloning, Molecular Cyclic AMP/metabolism Cyclic Nucleotide Phosphodiesterases, Type 1 DNA Mutational Analysis DNA Restriction Enzymes Gene Expression Regulation Genes, Fungal Hot Temperature Molecular Sequence Data Saccharomyces cerevisiae/genetics
Chemicals
Cyclic AMP DNA Restriction Enzymes 3',5'-Cyclic-AMP Phosphodiesterases Cyclic Nucleotide Phosphodiesterases, Type 1
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nikawa J
Cold Spring Harbor Laboratory, New York 11724.
Sass P
Wigler M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.10.3629-3636.1987
Published
1987-10-00
Pages
3629-36
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368017
Subset
IM
Databases
GENBANK
M17781
External Links
PubMed source DOI: 10.1128/mcb.7.10.3629-3636.1987 PMC Full Text View journal details
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