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PMID: 2824993 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Multiple basal elements of a human hsp70 promoter function differently in human and rodent cell lines.

Molecular and cellular biology ·Vol. 7 ·No. 10 ·1987-10-00 ·Pages 3646-55

Greene JM, Larin Z, Taylor IC, Prentice H, Gwinn KA, Kingston RE

Abstract

The human heat shock protein 70 (hsp70) gene is expressed constitutively in a wide variety of cells. Two separate promoter domains determine this basal level of hsp70 expression. The proximal domain is contained within 84 bases of the transcription initiation site and consists of three elements which appear to interact with the TATA factor(s) and CCAAT-box-binding transcription factor and SP1, respectively. The proximal domain is sufficient for near-maximal basal expression to rodent cell lines. The distal promoter domain consists of sequences upstream of -84 and is necessary in conjunction with the proximal domain for full basal expression in human cell lines. Although in BALB/c 3T3 cells the distal promoter domain plays little role in basal expression, it is functional as evidenced by the ability to compensate efficiently for mutations in the proximal CCAATC homology. The distal domain does not compensate as efficiently for proximal-domain mutations in HeLa cells. Basal expression of this human hsp70 promoter is, therefore, determined by multiple elements. Fewer elements are required for basal expression in rodent cell lines than in human cell lines, suggesting that there are significant differences between the rodent and human transcription apparatuses.

MeSH Terms
Animals Cell Line DNA Mutational Analysis DNA-Binding Proteins/physiology Endonucleases Gene Expression Regulation Heat-Shock Proteins/genetics Humans Methylation Mice Promoter Regions, Genetic Rats Regulatory Sequences, Nucleic Acid Single-Strand Specific DNA and RNA Endonucleases Species Specificity Transcription Factors/physiology
Chemicals
DNA-Binding Proteins Heat-Shock Proteins Transcription Factors Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Greene J M
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Larin Z
Taylor I C
Prentice H
Gwinn K A
Kingston R E
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.10.3646-3655.1987
Published
1987-10-00
Pages
3646-55
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368019
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.7.10.3646-3655.1987 PMC Full Text View journal details
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