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PMID: 2825137 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Determination of the single polyadenylation site of the human pro alpha 1(II) collagen gene.

Nucleic acids research ·Vol. 15 ·No. 22 ·1987-11-25 ·Pages 9499-504

Elima K, Vuorio T, Vuorio E

Abstract

Several cDNA clones for the human type II procollagen mRNA were isolated from a cartilage cDNA library. Six of the clones containing the longest inserts were subjected to restriction site mapping for alignment. All these six clones extended to the poly A tail. The longest clone, containing a 1470 bp insert, was named pHCAR3. Sequencing of pHCAR3 made it clear that neither of the two canonical AATAAA sequences of the human type II collagen gene is used as the polyadenylation signal. Two 60 bp stretches of high interspecies homology terminating in a hexanucleotide ATTAAA, located 23 nucleotides upstream of the poly A tail, apparently have an important role in determining the single polyadenylation signal for this gene. S1 protection experiments confirmed these observations.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/isolation & purification DNA Restriction Enzymes Fetus Genes Growth Plate/metabolism Humans Molecular Sequence Data Poly A/genetics,isolation & purification Procollagen/genetics
Chemicals
Procollagen Poly A DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Elima K
Department of Medical Biochemistry, University of Turku, Finland.
Vuorio T
Vuorio E
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21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.22.9499
Published
1987-11-25
Pages
9499-504
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306483
Subset
IM
Databases
GENBANK
X02664, X06268
External Links
PubMed source DOI: 10.1093/nar/15.22.9499 PMC Full Text View journal details
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