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PMID: 2825192 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The upstream activation site of a Ty2 element of yeast is necessary but not sufficient to promote maximal transcription of the element.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 23 ·1987-12-00 ·Pages 8520-4

Liao XB, Clare JJ, Farabaugh PJ

Abstract

The Ty elements of yeast are a family of retrovirus-like transposons that are highly transcribed, accounting for about 10% of total mRNA. We have mapped two sites to the nontranscribed region of the element upstream of the transcription start site that are required for maximal gene expression and are similar to sites previously defined in other genes. One, the TATA site, is located 74 base pairs upstream of the transcription start site and has the canonical sequence TATAAAA. This site is required for normal rates of initiation; deletion of the site greatly reduces the amount of Ty917 mRNA without changing its 5' end. A second site is located in a region from 140 to 110 base pairs upstream of the start site. Unlike other upstream activation site (UAS) elements previously defined, the Ty917 UAS is not sufficient to promote any transcription in the absence of downstream transcription regulatory sites. Thus the UAS is necessary but not sufficient for maximal transcription. Comparison of constructs lacking either the UAS or the downstream enhancer or both shows evidence of synergistic interaction between the sites since the effect of the sites on the rate of transcription initiation is more than additive.

MeSH Terms
DNA Transposable Elements Enhancer Elements, Genetic Gene Expression Regulation Genes, Fungal Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
DNA Transposable Elements
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Liao X B
Department of Microbiology, University of Connecticut Health Center, Farmington 06032.
Clare J J
Farabaugh P J
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.23.8520
Published
1987-12-00
Pages
8520-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299576
Subset
IM
Databases
GENBANK
M18805
External Links
PubMed source DOI: 10.1073/pnas.84.23.8520 PMC Full Text View journal details
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