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PMID: 2825658 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sex differences in the hydroxylation of cholecalciferol and of 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha-triol in rat liver.

The Biochemical journal ·Vol. 247 ·No. 1 ·1987-10-01 ·Pages 73-8

Saarem K, Pedersen JI

Abstract

The effect of sex hormones on hydroxylation of cholecalciferol ('vitamin D3') and of 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha-triol has been investigated in female- and male-rat livers. The mitochondrial cholecalciferol 25-hydroxylase and C27-steroid 27-hydroxylase activities were respectively 4.6- and 2.7-fold higher in female- than in male-rat livers. The microsomal 1 alpha-hydroxycholecalciferol 25-hydroxylase was 2.8-fold higher in male- than in female-rat liver. No significant difference was found in the microsomal 25-hydroxylation of 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha-triol. Liver microsomes (microsomal fractions) from male, but not from female, rats also catalysed 1-hydroxylation of 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha-triol. Injection of testosterone into female rats decreased the mitochondrial cholecalciferol 25-hydroxylase and C27-steroid 27-hydroxylase activities, but not to a statistically significant extent. Testosterone treatment had no effect on the microsomal hydroxylases in female-rat liver. Injection of oestradiol valerate to male rats resulted in increased activities of both mitochondrial hydroxylases to the same levels as those of control females, while the microsomal enzyme activities decreased. The present results indicate that sex hormones exert a regulatory control on the mitochondrial cholecalciferol 25-hydroxylase and C27-steroid 27-hydroxylase activities.

MeSH Terms
Animals Cholecalciferol/metabolism Cholestanols/metabolism Estradiol/analogs & derivatives,pharmacology Female Hydroxylation Liver/drug effects,metabolism Male Microsomes, Liver/metabolism Mitochondria, Liver/metabolism Rats Rats, Inbred Strains Sex Characteristics Starvation/metabolism Testosterone/pharmacology
Chemicals
Cholestanols Cholecalciferol Testosterone Estradiol 3,7,12-trihydroxycoprostane
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Saarem K
Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, Norway.
Pedersen J I
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2470073
Published
1987-10-01
Pages
73-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1148371
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2470073 PMC Full Text View journal details
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