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PMID: 2826125 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning and characterization of the mRNA for cyclin from sea urchin eggs.

The EMBO journal ·Vol. 6 ·No. 10 ·1987-10-00 ·Pages 2987-95

Pines J, Hunt T

Abstract

We have isolated a cDNA clone encoding sea urchin cyclin and determined its sequence. It contains a single open reading frame of 409 amino acids which shows homology with clam cyclins. RNA transcribed in vitro from this sequence was efficiently translated in reticulocyte lysates, yielding full-length cyclin. Injection of nanogram amounts of this synthetic mRNA into Xenopus oocytes caused them to mature more rapidly than with progesterone treatment. The sea urchin cyclin underwent two posttranslational modifications in the Xenopus oocytes during maturation. The first occurred at about the time that maturation became cycloheximide-resistant, when a small apparent increase in the molecular weight of cyclin was observed. The second modification involved destruction of the cyclin at about the time of white spot appearance, just as would have occurred at the metaphase/anaphase transition in the natural environment of a cleaving sea urchin embryo.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular Cyclins DNA/isolation & purification DNA Restriction Enzymes Embryo, Nonmammalian/physiology Female Genes Invertebrate Hormones/isolation & purification Molecular Sequence Data Nucleic Acid Hybridization Oocytes/physiology RNA, Messenger/genetics Sea Urchins
Chemicals
Cyclins Invertebrate Hormones RNA, Messenger DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pines J
University of Cambridge, Department of Biochemistry, UK.
Hunt T
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30 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-10-00
Pages
2987-95
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553735
Subset
IM
Databases
GENBANK
Y00608
External Links
PubMed source PMC Full Text View journal details
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