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PMID: 2826132 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Expression of proteins essential for IS1 transposition: specific binding of InsA to the ends of IS1.

The EMBO journal ·Vol. 6 ·No. 10 ·1987-10-00 ·Pages 3163-9

Zerbib D, Jakowec M, Prentki P, Galas DJ, Chandler M

Abstract

The insertion sequence IS1 displays a complex array of open reading frames (ORF). In an attempt to identify those which encode polypeptide products, we have systematically placed each ORF under the control of the P1 promoter of phage lambda. In the expression system we used, only the product of the insA gene was present in high enough amounts to be detected by polyacrylamide gel electrophoresis. The production of InsA was further increased in a first codon hook-up to phage T7 transcriptional and translational initiation signals. Cell extracts from InsA overproducers display a DNA binding activity specific for the ends of IS1. This activity was identified as the InsA protein itself.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Bacteriophage lambda/genetics Base Sequence Cloning, Molecular DNA Transposable Elements Escherichia coli/genetics Genes Genes, Viral Molecular Sequence Data Plasmids Promoter Regions, Genetic Protein Biosynthesis Transcription, Genetic
Chemicals
Bacterial Proteins DNA Transposable Elements
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zerbib D
Centre de Recherche de Biochimie et Genetique Cellulaire du C.N.R.S., Toulouse, France.
Jakowec M
Prentki P
Galas D J
Chandler M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-10-00
Pages
3163-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553758
Subset
IM
Grants
NIAID NIH HHS · AI1936 · United States
External Links
PubMed source PMC Full Text View journal details
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