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PMID: 2829117 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Infectivity and complete nucleotide sequence of the genome of a South African isolate of maize streak virus.

Nucleic acids research ·Vol. 16 ·No. 1 ·1988-01-11 ·Pages 229-49

Lazarowitz SG

Abstract

The complete infectious genome of a South African isolate of the geminivirus maize streak (MSV-S) has been cloned, characterized, and sequenced. Using an A. tumefaciens Ti plasmid delivery system, the cloned -2.7 kb single circular MSV component was shown to be necessary and sufficient for infection of maize. Based on sequence analysis of the infectious clone, MSV-S is highly homologous to the previously characterized Kenyan and Nigerian isolates. While the genomic organization of MSV-S has elements in common with each of these previously characterized isolates, it is identical to neither and its analysis addresses the discrepancies between them. The result is a somewhat simplified and unified picture of the viral genome, the structural organization of which is essentially identical to that of wheat dwarf virus.

MeSH Terms
Base Sequence Cloning, Molecular DNA Restriction Enzymes Genes, Viral Molecular Sequence Data Nucleic Acid Hybridization Plant Viruses/genetics South Africa Virion/genetics Zea mays
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Lazarowitz S G
Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210.
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.1.229
Published
1988-01-11
Pages
229-49
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334623
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.1.229 PMC Full Text View journal details
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