Home LiteratureArticle Details
PMID: 2829797 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human cytomegalovirus morphogenesis: an ultrastructural study of the late cytoplasmic phases.

Archives of virology ·Vol. 98 ·No. 1-2 ·1988-00-00 ·Pages 51-64

Severi B, Landini MP, Govoni E

Abstract

The late cytoplasmic phases of human Cytomegalovirus (HCMV) morphogenesis in cultured fibroblasts have been studied by transmission electron microscopy focusing attention on the relationship between the viral particles and host cell organelles. The results obtained largely reflect changes in cells subjected to sublethal injurious stimuli induced by many viruses as well as different noxious agents. A great increase in the number of Golgi apparatuses and lysosomes was observed, both of them interacting with the viral progeny. HCMV seems to acquire its final envelope from Golgi-derived structures and, less frequently, from the plasma membrane.

MeSH Terms
Biological Transport Cell Compartmentation Cell Line Cell Membrane/metabolism Cytomegalovirus/growth & development,ultrastructure Golgi Apparatus/ultrastructure Humans Microscopy, Electron Morphogenesis Virus Replication
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Severi B
Institute of Clinical Electron Microscopy, University of Bologna, Italy.
Landini M P
Govoni E
References (48)
48 references, click to expand
  1. Structural components of human cytomegalovirus: in situ localization of the major glycoprotein.
    Intervirology. 1987;27(3):154-60 PMID: 2822605
  2. Membrane differentiation within Golgi apparatus of rat hepatocytes.
    J Ultrastruct Res. 1979 Sep;68(3):317-24 PMID: 490759
  3. Monensin inhibits the processing of herpes simplex virus glycoproteins, their transport to the cell surface, and the egress of virions from infected cells.
    J Virol. 1982 Sep;43(3):1102-12 PMID: 6292453
  4. Segregation and packaging of granule enzymes in eosinophilic leukocytes.
    J Cell Biol. 1970 Apr;45(1):54-73 PMID: 5459000
  5. Ultrastructural studies of the envelopment and release of guinea pig herpes-like virus in cultured cells.
    Virology. 1973 Apr;52(2):468-77 PMID: 4735846
  6. Membrane fusion as a mechanism of simian virus 40 entry into different cellular compartments.
    J Virol. 1978 Dec;28(3):936-44 PMID: 215786
  7. Ultrastructural studies on the replication of herpes virus ateles-73 in owl monkey kidney cells.
    Arch Virol. 1979;60(1):59-73 PMID: 114147
  8. ULTRASTRUCTURAL, CYTOCHEMICAL, AND MICROCHEMICAL OBSERVATIONS ON CYTOMEGALOVIRUS (SALIVARY GLAND VIRUS) INFECTION OF HUMAN CELLS IN TISSUE CULTURE.
    Exp Mol Pathol. 1965 Feb;4:1-10 PMID: 14297550
  9. Cell injury with viruses.
    Am J Pathol. 1975 Oct;81(1):163-78 PMID: 1180330
  10. Autophagic vacuoles produced in vitro. I. Studies on cultured macrophages exposed to chloroquine.
    J Cell Biol. 1968 Aug;38(2):377-91 PMID: 4874491
  11. Nuclear envelope permeability.
    Nature. 1975 Mar 13;254(5496):109-14 PMID: 1117994
  12. Reduplication of nuclear membranes in tissue-culture cells infected with guinea-pig cytomegalovirus.
    Am J Pathol. 1967 May;50(5):779-90 PMID: 4290289
  13. Herpes simplex virus and human cytomegalovirus replication in WI-38 cells. I. Sequence of viral replication.
    J Virol. 1973 Oct;12(4):919-30 PMID: 4359959
  14. Ultrastructural development of guinea pig cytomegalovirus in cultured guinea pig embryo cells.
    J Gen Virol. 1979 Jan;42(1):127-40 PMID: 215704
  15. An ultrastructural investigation of cytomegalovirus replication in murine hepatocytes.
    J Gen Virol. 1984 Nov;65 ( Pt 11):1979-90 PMID: 6094712
  16. Concerning the egress of herpes simplex virus from infected cells: electron and light microscope observations.
    Virology. 1969 May;38(1):42-9 PMID: 4306525
  17. Release of infectious bovine rhinotracheitis virus from productively infected bovine kidney cells: an electron microscopic study.
    J Ultrastruct Res. 1972 Mar;38(5):433-43 PMID: 4335115
  18. Traffic control and structural proteins in the eukaryotic nucleus.
    Nature. 1985 Dec 12-18;318(6046):508-9 PMID: 4069225
  19. Human cytomegalovirus structural components: intracellular and intraviral localization of p28 and p65-69 by immunoelectron microscopy.
    Virus Res. 1987 Jul;8(1):15-23 PMID: 2821705
  20. A MORPHOLOGIC COMPARISON BETWEEN THE DEVELOPMENTAL STAGES OF HERPES ZOSTER AND HUMAN CYTOMEGALOVIRUS.
    Exp Mol Pathol. 1965 Feb;4:11-23 PMID: 14297547
  21. HISTOCHEMICAL STUDIES OF LYSOSOMES AND LYSOSOMAL ENZYMES IN VIRUS-INFECTED CELL CULTURES.
    J Exp Med. 1965 Mar 1;121:463-76 PMID: 14270244
  22. Observations on the growth of varicella-zoster virus in human diploid cells.
    J Gen Virol. 1973 Jan;18(1):21-31 PMID: 4354535
  23. Electron microscopic observations on FL cells infected with herpes simplex virus. II. Envelopment.
    Biken J. 1971 Sep;14(3):325-47 PMID: 4333479
  24. Observations on the mode of release of herpes virus from infected HeLa cells.
    J Cell Biol. 1962 Mar;12:589-97 PMID: 13890436
  25. Human cytomegalovirus: development and progression of nuclear inclusions by primary clinical isolates and laboratory-adapted strains.
    Proc Soc Exp Biol Med. 1986 Mar;181(3):416-22 PMID: 3003758
  26. Normal and 5-bromodeoxyuridine-inhibited development of herpes simplex virus. An electron microscope study.
    Exp Cell Res. 1966 Nov-Dec;44(2):241-55 PMID: 4290341
  27. Herpesvirus envelopment.
    J Virol. 1968 Jan;2(1):48-55 PMID: 4316013
  28. O-linked oligosaccharides are acquired by herpes simplex virus glycoproteins in the Golgi apparatus.
    Cell. 1983 Mar;32(3):987-97 PMID: 6299584
  29. Cytomegalovirus: nucleocapsid assembly and core structure.
    Intervirology. 1975;5(5):293-9 PMID: 178624
  30. Studies on cellular autophagocytosis. Relationship between heterophagy and autophagy in HeLa cells.
    Lab Invest. 1971 Feb;24(2):162-74 PMID: 5542737
  31. Human cytomegalovirus: glycoproteins associated with virions and dense bodies.
    J Virol. 1976 Aug;19(2):594-609 PMID: 183016
  32. A murine monoclonal antibody recognising a single glycoprotein within a human cytomegalovirus virion envelope glycoprotein complex.
    J Med Virol. 1985 Nov;17(3):255-66 PMID: 2999324
  33. Propagation in tissue cultures of a cytopathogenic virus from human salivary gland virus (SGV) disease.
    Proc Soc Exp Biol Med. 1956 Jun;92(2):424-30 PMID: 13350368
  34. MOUSE CYTOMEGALOVIRUS INFECTION. AN ELECTRON MICROSCOPIC STUDY OF HEPATIC PARENCHYMAL CELLS.
    Am J Pathol. 1964 May;44:799-821 PMID: 14155026
  35. Cytopathogenic agent resembling human salivary gland virus recovered from tissue cultures of human adenoids.
    Proc Soc Exp Biol Med. 1956 Jun;92(2):418-24 PMID: 13350367
  36. Cytomegalovirus: the relationship of nucleocapsid assembly to the organization of cellulae.
    Arch Virol. 1986;91(3-4):351-6 PMID: 3022685
  37. Herpes-type virus of the frog renal adenocarcinoma. I. Virus development in tumor transplants maintained at low temperature.
    J Virol. 1969 Jul;4(1):75-93 PMID: 5808113
  38. Replication of varicella-zoste virus in cell culture: an ultrastructural study.
    J Ultrastruct Res. 1970 Aug;32(3):334-50 PMID: 4318846
  39. Intracellular aspects of the process of protein synthesis.
    Science. 1975 Aug 1;189(4200):347-58 PMID: 1096303
  40. Isolation of intranuclear inclusion producing agents from infants with illnesses resembling cytomegalic inclusion disease.
    Proc Soc Exp Biol Med. 1957 Jan;94(1):4-12 PMID: 13400856
  41. Herpes simplex virus and human cytomegalovirus replication in WI-38 cells. III. Cytochemical localization of lysosomal enzymes in infected cells.
    J Virol. 1978 Apr;26(1):102-9 PMID: 206717
  42. Cytomegalovirus: an ultrastructural study of the morphogenesis of nuclear inclusions in human cell culture.
    J Gen Virol. 1981 Sep;56(Pt 1):97-104 PMID: 6271919
  43. Studies on the morphogenesis of murine cytomegalovirus.
    Intervirology. 1986;26(4):192-201 PMID: 3038772
  44. Replication of canine herpesvirus. II. Virus development and release in infected dog kidney cells.
    J Virol. 1969 Oct;4(4):480-9 PMID: 4309882
  45. Similarities and Differences in the Development of Laboratory Strains and Freshly Isolated Strains of Herpes Simplex Virus in HEp-2 Cells: Electron Microscopy.
    J Virol. 1969 Dec;4(6):879-89 PMID: 16789121
  46. Electron microscopic observations on the development of herpes simplex virus.
    J Exp Med. 1959 Oct 1;110:643-56 PMID: 14424096
  47. An additional role for the outer nuclear membrane in the morphogenesis of herpes simplex virus.
    Intervirology. 1980;13(5):312-6 PMID: 6250993
  48. HUMAN CYTOMEGALOVIRUS INFECTION. ELECTRON MICROSCOPIC AND HISTOCHEMICAL CHANGES IN CULTURES OF HUMAN FIBROBLASTS.
    Am J Pathol. 1965 Mar;46:477-96 PMID: 14266222
Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01321005
Published
1988-00-00
Pages
51-64
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01321005 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2