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PMID: 2830596 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Homologous recombination of SV40 DNA in COS7 cells occurs with high frequency in a gene dose independent fashion.

Nucleic acids research ·Vol. 16 ·No. 3 ·1988-02-11 ·Pages 941-52

Rommerskirch W, Graeber I, Grässmann M, Grässmann A

Abstract

Homologous recombination between microinjected SV40 DNA fragments and endogenous SV40 DNA in COS7 cells was analysed by immunofluorescence staining and DNA blotting. Time course experiments revealed that recombination between the transferred (trans) DNA and the chromosomal DNA occurred about 8 hours after microinjection with high efficiency in a gene dose independent fashion. Deletions of up to 1018 basepairs (bp) within the early or the late SV40 region were efficiently repaired after the transfer of linear but not of circular DNA molecules. A 22 bp homology between the trans DNA and the endogenous DNA was sufficient to initiate recombination but 14 nonhomologous bp at one open end of the SV40 DNA fragments hindered gap repair.

MeSH Terms
Animals Cell Line Chlorocebus aethiops DNA, Circular DNA, Recombinant DNA, Viral/metabolism Fibroblasts Microinjections Recombination, Genetic Sequence Homology, Nucleic Acid Simian virus 40/genetics
Chemicals
DNA, Circular DNA, Recombinant DNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rommerskirch W
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, FRG.
Graeber I
Grässmann M
Grässmann A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.3.941
Published
1988-02-11
Pages
941-52
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334729
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.3.941 PMC Full Text View journal details
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