Home LiteratureArticle Details
PMID: 2831503 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The genetic structure of mouse ornithine transcarbamylase.

Nucleic acids research ·Vol. 16 ·No. 4 ·1988-02-25 ·Pages 1593-601

Scherer SE, Veres G, Caskey CT

Abstract

The gene encoding the mouse urea cycle enzyme, ornithine transcarbamylase has been isolated on five partially overlapping bacteriophage lambda clones. We have characterized the gene and found that it is split between ten exons distributed over approximately 70 kb of the X chromosome. The introns range in size from 88 bases to the relatively unusual size of approximately 26 kilobases, while the splice donor/splice acceptor sequences conform to the consensus established for other eukaryotic genes.

MeSH Terms
Animals Base Sequence DNA Restriction Enzymes DNA, Recombinant/metabolism Genes Mice Molecular Sequence Data Nucleic Acid Hybridization Ornithine Carbamoyltransferase/genetics
Chemicals
DNA, Recombinant Ornithine Carbamoyltransferase DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Scherer S E
Institute for Molecular Genetics, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030.
Veres G
Caskey C T
References (25)
25 references, click to expand
  1. Citrulline synthesis in rat tissues and liver content of carbamoyl phosphate and ornithine.
    Biochem J. 1974 Feb;138(2):225-32 PMID: 4822731
  2. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  3. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  4. In vitro synthesis of a putative precursor of mitochondrial ornithine transcarbamoylase.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5724-7 PMID: 293676
  5. The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper.
    Anal Biochem. 1980 Nov 15;109(1):123-9 PMID: 6162401
  6. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  7. Ornithine transcarbamylase in liver mitochondria.
    Mol Cell Biochem. 1982 Nov 26;49(2):97-111 PMID: 6759918
  8. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  9. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  10. Structure and expression of a complementary DNA for the nuclear coded precursor of human mitochondrial ornithine transcarbamylase.
    Science. 1984 Jun 8;224(4653):1068-74 PMID: 6372096
  11. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
    Anal Biochem. 1984 Feb;137(1):266-7 PMID: 6329026
  12. Human ornithine transcarbamylase locus mapped to band Xp21.1 near the Duchenne muscular dystrophy locus.
    Science. 1984 Nov 9;226(4675):698-700 PMID: 6494904
  13. Molecular cloning and nucleotide sequence of cDNA for rat ornithine carbamoyltransferase precursor.
    Proc Natl Acad Sci U S A. 1984 Dec;81(23):7412-6 PMID: 6095294
  14. Rapid restriction mapping of DNA cloned in lambda phage vectors.
    Gene. 1984 Oct;30(1-3):195-200 PMID: 6096214
  15. Removal of repeated sequences from hybridisation probes.
    Nucleic Acids Res. 1985 Mar 25;13(6):1905-22 PMID: 4000947
  16. Expression of nuclear genes encoding the urea cycle enzymes, carbamoyl-phosphate synthetase I and ornithine carbamoyl transferase, in rat liver and intestinal mucosa.
    Eur J Biochem. 1985 Oct 15;152(2):287-92 PMID: 2865132
  17. Targeting of pre-ornithine transcarbamylase to mitochondria: definition of critical regions and residues in the leader peptide.
    Cell. 1986 Feb 14;44(3):451-9 PMID: 3943133
  18. The 5' flanking region of the ornithine transcarbamylase gene contains DNA sequences regulating tissue-specific expression.
    J Biol Chem. 1986 Jun 15;261(17):7588-91 PMID: 3011788
  19. Diversity of alpha-fetoprotein gene expression in mice is generated by a combination of separate enhancer elements.
    Science. 1987 Jan 2;235(4784):53-8 PMID: 2432657
  20. The molecular basis of the sparse fur mouse mutation.
    Science. 1987 Jul 24;237(4813):415-7 PMID: 3603027
  21. Structure of the rat ornithine carbamoyltransferase gene, a large, X chromosome-linked gene with an atypical promoter.
    Proc Natl Acad Sci U S A. 1987 Sep;84(17):6136-40 PMID: 3476935
  22. The catalytic rôle of carbamyl glutamate in citrulline biosynthesis.
    J Biol Chem. 1952 Oct;198(2):561-71 PMID: 12999771
  23. Citrulline synthesis in rat tissues.
    Arch Biochem Biophys. 1961 Dec;95:499-507 PMID: 14452306
  24. Abnormal ornithine carbamoyltransferase in mice having the sparse-fur mutation.
    Proc Natl Acad Sci U S A. 1976 May;73(5):1693-7 PMID: 5727
  25. A new allele of the sparse fur gene in the mouse.
    J Hered. 1974 May-Jun;65(3):194-5 PMID: 4603259
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.4.1593
Published
1988-02-25
Pages
1593-601
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336337
Subset
IM
Grants
NICHD NIH HHS · HD21452 · United States
Databases
GENBANK
X07092, X07093, X07094, X07095, X07096, X07097, X07098, X07099, X07100
External Links
PubMed source DOI: 10.1093/nar/16.4.1593 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2