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PMID: 2833740 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Partial amino acid sequence of porcine 1,25-dihydroxyvitamin D3 receptor isolated by immunoaffinity chromatography.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 8 ·1988-04-00 ·Pages 2454-8

Brown TA, Prahl JM, DeLuca HF

Abstract

Monoclonal antibodies against the porcine 1,25-dihydroxyvitamin D3 receptor were immobilized on Sepharose CL-4B and used to obtain a highly purified 1,25-dihydroxyvitamin D3 receptor fraction with a 45% recovery of the 1,25-dihydroxyvitamin D3 binding capacity. The porcine receptor was purified to homogeneity by preparative electrophoresis and digested in sodium dodecyl sulfate/polyacrylamide gels with Staphylococcus aureus strain V8 protease. The resulting peptides were separated by sodium dodecyl sulfate/polyacrylamide gel electrophoresis, electrophoretically transferred to polyvinylidene difluoride membranes, and directly sequenced. The generation and isolation of peptides by this method allows sequencing of proteins present in trace amounts as well as those whose amino termini have been modified. The 1,25-dihydroxyvitamin D3 receptor amino acid sequence corresponded to the sequence predicted from a recently cloned receptor cDNA obtained from rat kidney mRNAs.

MeSH Terms
Amino Acid Sequence Animals Calcitriol/metabolism Chromatography, Affinity Molecular Sequence Data Peptide Mapping Receptors, Calcitriol Receptors, Steroid/isolation & purification Swine
Chemicals
Receptors, Calcitriol Receptors, Steroid Calcitriol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brown T A
Department of Biochemistry, University of Wisconsin, College of Agricultural and Life Sciences, Madison 53706.
Prahl J M
DeLuca H F
References (26)
26 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Isolation and expression of rat 1,25-dihydroxyvitamin D3 receptor cDNA.
    Proc Natl Acad Sci U S A. 1988 Feb;85(4):1005-9 PMID: 2829212
  3. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  4. An hydroxylapatite batch assay for the quantitation of 1alpha,25-dihydroxyvitamin D3-receptor complexes.
    Anal Biochem. 1979 Jan 15;92(2):314-23 PMID: 443532
  5. Direct chemical synthesis of 1 alpha,25-dihydroxy[26,27-3H]vitamin D3 with high specific activity: its use in receptor studies.
    Biochemistry. 1980 May 27;19(11):2515-21 PMID: 6892993
  6. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.
    Anal Biochem. 1980 Jul 1;105(2):361-3 PMID: 6161559
  7. Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.
    Anal Biochem. 1980 Nov 15;109(1):76-86 PMID: 6258458
  8. Purification of chicken intestinal receptor for 1 alpha, 25-dihydroxyvitamin D3 to apparent homogeneity.
    Proc Natl Acad Sci U S A. 1982 Jan;79(1):16-20 PMID: 6275386
  9. A new approach (cyano-transfer) for cyanogen bromide activation of Sepharose at neutral pH, which yields activated resins, free of interfering nitrogen derivatives.
    Biochem Biophys Res Commun. 1982 Aug;107(3):878-84 PMID: 7138526
  10. A new solid-state reagent to iodinate proteins. I. Conditions for the efficient labeling of antiserum.
    Anal Biochem. 1982 Sep 15;125(2):427-32 PMID: 6758629
  11. Serum and monoclonal antibodies against the chick intestinal receptor for 1,25-dihydroxyvitamin D3. Generation by a preparation enriched in a 64,000-dalton protein.
    J Biol Chem. 1983 Jan 25;258(2):1289-96 PMID: 6296076
  12. A high recovery method for concentrating microgram quantities of protein from large volumes of solution.
    Anal Biochem. 1983 Mar;129(2):513-6 PMID: 6846845
  13. Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis.
    Methods Enzymol. 1983;91:227-36 PMID: 6855576
  14. Vitamin D: recent advances.
    Annu Rev Biochem. 1983;52:411-39 PMID: 6311080
  15. Identification of the porcine intestinal 1,25-dihydroxyvitamin D3 receptor on sodium dodecyl sulfate/polyacrylamide gels by renaturation and immunoblotting.
    Proc Natl Acad Sci U S A. 1985 Dec;82(23):7825-9 PMID: 2999778
  16. Steroid hormone receptors and the nucleus.
    Endocr Rev. 1985 Fall;6(4):512-43 PMID: 3000755
  17. Steroid receptor regulated transcription of specific genes and gene networks.
    Annu Rev Genet. 1985;19:209-52 PMID: 3909942
  18. Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea.
    Anal Biochem. 1986 May 15;155(1):83-8 PMID: 3454661
  19. Monoclonal antibodies to the porcine intestinal receptor for 1,25-dihydroxyvitamin D3: interaction with distinct receptor domains.
    Biochemistry. 1986 Aug 12;25(16):4523-34 PMID: 2429689
  20. Chicken intestinal receptor for 1,25-dihydroxyvitamin D3. Immunologic characterization and homogeneous isolation of a 60,000-dalton protein.
    J Biol Chem. 1987 Jan 25;262(3):1305-11 PMID: 3027085
  21. Immunochemical detection of unique proteolytic fragments of the chick 1,25-dihydroxyvitamin D3 receptor. Distinct 20-kDa DNA-binding and 45-kDa hormone-binding species.
    J Biol Chem. 1987 Jan 25;262(3):1312-9 PMID: 3027086
  22. Molecular cloning of complementary DNA encoding the avian receptor for vitamin D.
    Science. 1987 Mar 6;235(4793):1214-7 PMID: 3029866
  23. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.
    J Biol Chem. 1987 Jul 25;262(21):10035-8 PMID: 3611052
  24. Molecular cloning of the cDNA and chromosomal gene for vitamin D-dependent calcium-binding protein of rat intestine.
    Proc Natl Acad Sci U S A. 1987 Sep;84(17):6108-11 PMID: 3476932
  25. Size and charge of the functional 1,25-dihydroxyvitamin D receptor in porcine intestine.
    J Biol Chem. 1987 Dec 15;262(35):17092-9 PMID: 2824516
  26. Equilibrium binding of estradiol by uterine cell suspensions and whole uteri in vitro.
    Biochemistry. 1974 Dec 31;13(27):5537-42 PMID: 4457113
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.8.2454
Published
1988-04-00
Pages
2454-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC280015
Subset
IM
Grants
NIDDK NIH HHS · DK-14881 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.8.2454 PMC Full Text View journal details
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