Asymmetric budding of viruses in epithelial monlayers: a model system for study of epithelial polarity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 10 ·1978-10-00 ·Pages 5071-5

Rodriguez Boulan E, Sabatini DD

Abstract

Infection of two different lines of polarized epithelial cells grown as monolayers with several types of enveloped viruses results, for each virus type, in a characteristic asymmetric budding of virions. Influenza virus (WSN strain), simian virus 5, and Sendai virus bud exclusively from the free (apical) surface of the cells, while vesicular stomatitis virus acquires its envelope only from the basolateral plasma membrane. Because different viruses select specific domains of plasma membrane in the same cell type, virus-infected epithelial monolayers can provide an excellent model system for studies of the mechanisms that generate regional differences in the distribution of plasma membrane components of epithelial cells.

MeSH Terms

Cell Line Cell Membrane/metabolism Epithelium/metabolism,microbiology Membrane Proteins/metabolism Models, Biological Morphogenesis Viruses/growth & development

Chemicals

Membrane Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Rodriguez Boulan E

Sabatini D D

References (14)

14 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.10.5071

Published

1978-10-00

Pages

5071-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC336265

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.10.5071 PMC Full Text View journal details

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Asymmetric budding of viruses in epithelial monlayers: a model system for study of epithelial polarity.

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