Restriction endonuclease analysis of DNA from Chlamydia trachomatis biovars.
DNA from a total of 60 Chlamydia trachomatis isolates was examined by restriction endonuclease analysis. Strains from all established biovars and serovars were tested. There was great diversity between the mouse biovar and the lymphogranuloma venereum (LGV) and trachoma biovars. The LGV and trachoma biovar isolates generated similar fragment patterns; however, distinct fragments appeared to be unique to both biovars, thus allowing differentiation of these two major groups. In most cases, strains of the same serovar could be differentiated from one another when a battery of restriction enzymes was used. In addition, in some cases, certain restriction fragments appeared to be characteristic of strains from a particular geographical location. The DNA patterns generated by all C. trachomatis isolates differed greatly from the DNA patterns generated from the Chlamydia psittaci isolates tested, including TWAR, a human C. psittaci strain.
17 references, click to expand
-
Immunotyping of Chlamydia trachomatis with monoclonal antibodies.J Infect Dis. 1985 Oct;152(4):791-800 PMID: 4045232
-
Isolation and characterization of deletion mutants of herpes simplex virus type 2 (strain HG52).J Gen Virol. 1985 Jun;66 ( Pt 6):1305-21 PMID: 2989417
-
Selective extraction of polyoma DNA from infected mouse cell cultures.J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
-
Cloned, random chromosomal sequences as probes to identify Salmonella species.J Infect Dis. 1986 Jul;154(1):156-62 PMID: 3011919
-
Genetic structure of populations of Legionella pneumophila.J Bacteriol. 1985 Sep;163(3):1021-37 PMID: 4030689
-
Molecular characterization of Chlamydia trachomatis and Chlamydia psittaci plasmids.Infect Immun. 1986 Feb;51(2):699-703 PMID: 3943908
-
Anatomy of herpes simplex virus DNA: strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites.Proc Natl Acad Sci U S A. 1975 May;72(5):1768-72 PMID: 168574
-
Differential amino acid utilization by Chlamydia psittaci (strain guinea pig inclusion conjunctivitis) and its regulatory effect on chlamydial growth.J Gen Microbiol. 1983 Jul;129(7):1991-2000 PMID: 6415225
-
Characterization of the new Chlamydia agent, TWAR, as a unique organism by restriction endonuclease analysis and DNA-DNA hybridization.J Clin Microbiol. 1987 Oct;25(10):1911-6 PMID: 2822763
-
A new Chlamydia psittaci strain, TWAR, isolated in acute respiratory tract infections.N Engl J Med. 1986 Jul 17;315(3):161-8 PMID: 3724806
-
Deoxyribonucleic Acid Heterogeneity Between Human and Murine Strains of Chlamydia trachomatis.Infect Immun. 1970 Jul;2(1):24-8 PMID: 16557794
-
Cultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells.J Clin Microbiol. 1977 Oct;6(4):328-31 PMID: 562356
-
Characterization of Chlamydia DNA by restriction endonuclease cleavage.Infect Immun. 1983 Aug;41(2):604-8 PMID: 6307876
-
Immunologic relationship between genital TRIC, lymphogranuloma venereum, and related organisms in a new microtiter indirect immunofluorescence test.Am J Ophthalmol. 1970 Sep;70(3):367-74 PMID: 4915925
-
Differences in outer membrane proteins of the lymphogranuloma venereum and trachoma biovars of Chlamydia trachomatis.Infect Immun. 1985 Nov;50(2):488-94 PMID: 4055030
-
Comparison of Chlamydia psittaci isolates by DNA restriction endonuclease analysis.Infect Immun. 1984 Aug;45(2):384-9 PMID: 6086526
-
Lack of deoxyribonucleic acid homology between species of the genus Chlamydia.J Bacteriol. 1968 Oct;96(4):1421-3 PMID: 5686006
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
