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PMID: 2835637 Published · ppublish English Journal Article

Expression of tobacco mosaic virus RNA in transgenic plants.

Molecular & general genetics : MGG ·Vol. 211 ·No. 3 ·1988-03-00 ·Pages 520-5

Yamaya J, Yoshioka M, Meshi T, Okada Y, Ohno T

Abstract

Tobacco mosaic virus (TMV) is a message-sense, single-stranded RNA virus that infects many Solanaceae plants. A full-length cDNA copy of TMV genomic RNA was constructed and introduced into the genomic DNA of tobacco plants using a disarmed Ti plasmid vector. Transformed plants showed typical symptoms of TMV infection, and their leaves contained infectious TMV particles. This is the first example of the expression of RNA virus genomic RNAs in plants.

MeSH Terms
Base Sequence DNA Restriction Enzymes Genes, Viral Genetic Engineering Molecular Sequence Data Plants/genetics,microbiology Plants, Toxic Promoter Regions, Genetic RNA, Viral/genetics Tobacco/genetics,microbiology Tobacco Mosaic Virus/genetics Transcription, Genetic
Chemicals
RNA, Viral DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Yamaya J
Plant Laboratory, Kirin Brewery Co., Tochigi, Japan.
Yoshioka M
Meshi T
Okada Y
Ohno T
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22 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425710
Published
1988-03-00
Pages
520-5
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00425710
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