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PMID: 2835660 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cap-independent translation of poliovirus mRNA is conferred by sequence elements within the 5' noncoding region.

Molecular and cellular biology ·Vol. 8 ·No. 3 ·1988-03-00 ·Pages 1103-12

Pelletier J, Kaplan G, Racaniello VR, Sonenberg N

Abstract

Poliovirus polysomal RNA is naturally uncapped, and as such, its translation must bypass any 5' cap-dependent ribosome recognition event. To elucidate the manner by which poliovirus mRNA is translated, we have determined the translational efficiencies of a series of deletion mutants within the 5' noncoding region of the mRNA. We found striking differences in translatability among the altered mRNAs when assayed in mock-infected and poliovirus-infected HeLa cell extracts. The results identify a functional cis-acting element within the 5' noncoding region of the poliovirus mRNA which enables it to translate in a cap-independent fashion. The major determinant of this element maps between nucleotides 320 and 631 of the 5' end of the poliovirus mRNA. We also show that this region (320 to 631), when fused to a heterologous mRNA, can function in cis to render the mRNA cap independent in translation.

MeSH Terms
Base Sequence Cloning, Molecular Genes, Viral HeLa Cells Humans Mutation Poliovirus/genetics Protein Biosynthesis RNA Caps/genetics RNA, Messenger/genetics RNA, Viral/genetics Templates, Genetic Transcription, Genetic
Chemicals
RNA Caps RNA, Messenger RNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pelletier J
Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
Kaplan G
Racaniello V R
Sonenberg N
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32 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.3.1103-1112.1988
Published
1988-03-00
Pages
1103-12
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363253
Subset
IM
Grants
NIAID NIH HHS · AI-20017 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.3.1103-1112.1988 PMC Full Text View journal details
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