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PMID: 2835674 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Separation of simian virus 40 large-T-antigen-transforming and origin-binding functions from the ability to block differentiation.

Molecular and cellular biology ·Vol. 8 ·No. 3 ·1988-03-00 ·Pages 1380-4

Cherington V, Brown M, Paucha E, St Louis J, Spiegelman BM, Roberts TM

Abstract

Wild-type simian virus 40 large T antigen is very effective at blocking adipocyte differentiation in 3T3-F442A cells as assayed by triglyceride accumulation, induction of glycerophosphate dehydrogenase activity, and expression of mRNAs for glycerophosphate dehydrogenase, the adipocyte serine protease adipsin, and the putative lipid-binding protein adipocyte P2. Point mutants defective for either origin-specific DNA binding or transformation blocked differentiation as completely as wild type.

MeSH Terms
Adipose Tissue/cytology Antigens, Polyomavirus Transforming/genetics,immunology Cell Differentiation Cell Line Fluorescent Antibody Technique Gene Expression Regulation Immunoassay Mutation Oncogenes Simian virus 40/genetics,immunology
Chemicals
Antigens, Polyomavirus Transforming
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cherington V
Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111.
Brown M
Paucha E
St Louis J
Spiegelman B M
Roberts T M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.3.1380-1384.1988
Published
1988-03-00
Pages
1380-4
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363287
Subset
IM
Grants
NIADDK NIH HHS · AM31405 · United States
NCI NIH HHS · CA30002 · United States
NCRR NIH HHS · S07-RR05598-21 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.3.1380-1384.1988 PMC Full Text View journal details
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