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PMID: 2838744 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cooperation between the polyomavirus middle-T-antigen gene and the human c-myc oncogene in a rat thyroid epithelial differentiated cell line: model of in vitro progression.

Molecular and cellular biology ·Vol. 8 ·No. 5 ·1988-05-00 ·Pages 2261-6

Berlingieri MT, Portella G, Grieco M, Santoro M, Fusco A

Abstract

Two rat thyroid epithelial differentiated cell lines, PC Cl 3 and PC myc, were infected with the polyoma murine leukemia virus (PyMLV) carrying the Middle-T-antigen gene of polyomavirus. After infection, both cell lines acquired the typical markers of neoplastic transformation; however, the PC myc cells showed a greater malignant phenotype. Furthermore, the thyroid differentiated functions were completely suppressed in PC myc cells transformed by PyMLV, whereas they were, at least partially, retained in PC Cl 3 cells transformed by PyMLV, and in particular, thyroglobulin synthesis and secretion were not affected at all. Since no differences in the expression of the middle-T-antigen gene were observed in the two PyMLV-transformed cell lines, the different properties shown by these two infected cell lines must be ascribed to the expression of the c-myc oncogene.

MeSH Terms
Animals Antigens, Polyomavirus Transforming/physiology Cell Line Cell Transformation, Neoplastic/genetics Epithelium Genes, Viral Humans Oncogenes Polyomavirus/genetics,physiology Proto-Oncogene Proteins/physiology Proto-Oncogene Proteins c-myc Rats Rats, Inbred F344 Thyroglobulin/metabolism Thyroid Gland
Chemicals
Antigens, Polyomavirus Transforming Proto-Oncogene Proteins Proto-Oncogene Proteins c-myc Thyroglobulin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Berlingieri M T
Dipartimento di Biologia e Patologia Cellulare e Molecolare, II Facoltà di Medicina e Chirurgia, Università di Napoli, Italy.
Portella G
Grieco M
Santoro M
Fusco A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.5.2261-2266.1988
Published
1988-05-00
Pages
2261-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363414
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.8.5.2261-2266.1988 PMC Full Text View journal details
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