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PMID: 2839695 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Activity of avian retroviral protease expressed in Escherichia coli.

Journal of virology ·Vol. 62 ·No. 8 ·1988-08-00 ·Pages 2696-700

Kotler M, Katz RA, Skalka AM

Abstract

The 3' end of the avian sarcoma leukosis virus (ASLV) gag gene encodes a 124-amino-acid protease (PR) responsible for processing the gag and pol polyprotein precursors into the mature virion structural proteins and the reverse transcriptase. Here we report the synthesis of the mature ASLV PR and a nucleocapsid (NC)-PR gag precursor fragment in Escherichia coli. E. coli extracts containing mature PR correctly cleaved a synthetic decapeptide homologous to a known ASLV cleavage site. Also, the NC-PR precursor fragment appeared to be correctly processed to produce NC and PR in the bacterial cells. This cleavage was blocked by a mutation in the putative active site of PR. These results strongly support the hypothesis that PR is involved in cleaving itself from the gag precursor.

MeSH Terms
Aspartic Acid Avian Sarcoma Viruses/enzymology,genetics Capsid/genetics DNA Mutational Analysis DNA, Recombinant Escherichia coli Gene Expression Regulation Gene Products, gag Peptide Hydrolases/genetics,metabolism Protein Processing, Post-Translational Retroviridae Proteins/genetics Viral Core Proteins/genetics Viral Proteins/metabolism
Chemicals
DNA, Recombinant Gene Products, gag Retroviridae Proteins Viral Core Proteins Viral Proteins Aspartic Acid Peptide Hydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kotler M
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Katz R A
Skalka A M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.8.2696-2700.1988
Published
1988-08-00
Pages
2696-700
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253702
Subset
IM
Grants
NCI NIH HHS · CA-06927 · United States
NCRR NIH HHS · RR-05539 · United States
External Links
PubMed source DOI: 10.1128/JVI.62.8.2696-2700.1988 PMC Full Text View journal details
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