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PMID: 2840639 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes.

Nucleic acids research ·Vol. 16 ·No. 13 ·1988-07-11 ·Pages 6015-25

Pyle LE, Corcoran LN, Cocks BG, Bergemann AD, Whitley JC, Finch LR

Abstract

The sizes of large DNA fragments produced from genomes of members of the Mycoplasmataceae by digestion with restriction endonucleases having infrequent (1 to 3) cleavage sites within the genome were estimated from their mobility in contour-clamped homogeneous electric field (CHEF) agarose gel electrophoresis by comparison with yeast chromosomal DNA markers. The estimates of total genome size for 7 strains of 6 species ranged from approximately 900 kilo base pairs (kb) for Ureaplasma urealyticum 960T to 1330 kb for M. mycoides subsp. mycoides, GC-1176. The values derived from this new method are considerably higher than those of approximately 500 Mdaltons or 750 kb previously reported for genome sizes in members of the Mycoplasmataceae.

MeSH Terms
DNA Restriction Enzymes/metabolism DNA, Fungal/analysis Deoxyribonuclease BamHI Electrophoresis, Agar Gel/methods Genes, Fungal Genetic Markers Mycoplasmataceae/genetics
Chemicals
DNA, Fungal Genetic Markers DNA Restriction Enzymes Deoxyribonuclease BamHI
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Pyle L E
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
Corcoran L N
Cocks B G
Bergemann A D
Whitley J C
Finch L R
References (19)
19 references, click to expand
  1. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.
    Nucleic Acids Res. 1984 Jul 25;12(14):5647-64 PMID: 6379602
  2. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.
    Cell. 1984 May;37(1):67-75 PMID: 6373014
  3. Size variation in chromosomes from independent cultured isolates of Plasmodium falciparum.
    Nature. 1985 May 23-29;315(6017):347-50 PMID: 3889657
  4. Genetic map of Saccharomyces cerevisiae, edition 9.
    Microbiol Rev. 1985 Sep;49(3):181-213 PMID: 2995780
  5. Molecular biology and genetics of mycoplasmas (Mollicutes).
    Microbiol Rev. 1985 Dec;49(4):419-55 PMID: 3912655
  6. Electrophoretic separations of large DNA molecules by periodic inversion of the electric field.
    Science. 1986 Apr 4;232(4746):65-8 PMID: 3952500
  7. Nucleotide sequence and genetic map of the 16-kb vaccinia virus HindIII D fragment.
    Virology. 1986 Aug;153(1):96-112 PMID: 3739227
  8. Separation of large DNA molecules by contour-clamped homogeneous electric fields.
    Science. 1986 Dec 19;234(4783):1582-5 PMID: 3538420
  9. Chromosome analysis by two-dimensional fingerprinting.
    Gene. 1986;49(1):93-102 PMID: 3032749
  10. Restriction endonucleases for pulsed field mapping of bacterial genomes.
    Nucleic Acids Res. 1987 Aug 11;15(15):5985-6005 PMID: 2819819
  11. Resolution of DNA molecules greater than 5 megabases by contour-clamped homogeneous electric fields.
    Nucleic Acids Res. 1987 Oct 12;15(19):7865-76 PMID: 2959907
  12. Preparation and FIGE separation of infrequent restriction fragments from Mycoplasma mycoides DNA.
    Nucleic Acids Res. 1988 Mar 25;16(5):2263-8 PMID: 2833729
  13. Kinetics of renaturation of DNA.
    J Mol Biol. 1968 Feb 14;31(3):349-70 PMID: 5637197
  14. Genome size of mycoplasmal DNA.
    Nature. 1969 Dec 20;224(5225):1209-10 PMID: 5358346
  15. Cell biology of the mycoplasmas.
    Bacteriol Rev. 1972 Sep;36(3):263-90 PMID: 4345848
  16. Analysis of repeating DNA sequences by reassociation.
    Methods Enzymol. 1974;29:363-418 PMID: 4850571
  17. Enzymes of purine metabolism in Mycoplasma mycoides subsp. mycoides.
    J Bacteriol. 1978 Jun;134(3):706-12 PMID: 207675
  18. Restriction enzyme mapping of vaccinia virus DNA.
    J Virol. 1982 Jul;43(1):136-49 PMID: 6286993
  19. Characterization of the mycoplasma genome.
    Yale J Biol Med. 1983 Sep-Dec;56(5-6):357-66 PMID: 6206652
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.13.6015
Published
1988-07-11
Pages
6015-25
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336844
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.13.6015 PMC Full Text View journal details
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