Home LiteratureArticle Details
PMID: 2841584 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transposable element-mediated enhancement of gene expression in Saccharomyces cerevisiae involves sequence-specific binding of a trans-acting factor.

Molecular and cellular biology ·Vol. 8 ·No. 6 ·1988-06-00 ·Pages 2572-80

Goel A, Pearlman RE

Abstract

In our studies on the regulation of adjacent-gene expression by Ty sequences, we demonstrated that a single-base-pair change (T-A----C-G) in the epsilon sequence of Ty917-derived elements is primarily responsible for enhancement of beta-galactosidase expression from lacZ fusion plasmids. Using an electrophoretic gel mobility assay, we showed that the same base pair transition is required for binding of a trans-acting factor, TyBF, from crude cell extracts in vitro. We identified the site of TyBF binding and determined the guanine nucleotide contact sites required for TyBF interaction. We propose that TyBF binding to cis-acting Ty2 sequences activates adjacent-gene transcription.

MeSH Terms
Base Sequence Binding, Competitive Conjugation, Genetic DNA/analysis,metabolism DNA Transposable Elements DNA-Binding Proteins/metabolism Enhancer Elements, Genetic Fungal Proteins/metabolism Gene Expression Regulation Methylation Plasmids Saccharomyces cerevisiae/genetics Transcription Factors/metabolism
Chemicals
DNA Transposable Elements DNA-Binding Proteins Fungal Proteins Transcription Factors DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Goel A
Department of Biology, York University, Toronto, Ontario, Canada.
Pearlman R E
References (34)
34 references, click to expand
  1. Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2199-203 PMID: 6264467
  2. Mating signals control expression of mutations resulting from insertion of a transposable repetitive element adjacent to diverse yeast genes.
    Cell. 1980 Nov;22(2 Pt 2):427-36 PMID: 6256080
  3. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
    Nucleic Acids Res. 1981 Dec 11;9(23):6505-25 PMID: 6275366
  4. The nucleotide sequence of the HIS4 region of yeast.
    Gene. 1982 Apr;18(1):47-59 PMID: 7049842
  5. Movement of yeast transposable elements by gene conversion.
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5621-5 PMID: 6291054
  6. RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2432-6 PMID: 6189122
  7. Essential contact residues within SV40 large T antigen binding sites I and II identified by alkylation-interference.
    Cell. 1984 Jan;36(1):155-62 PMID: 6319005
  8. Preferential integration of yeast transposable element Ty into a promoter region.
    Nature. 1984 Jan 26-Feb 1;307(5949):386-8 PMID: 6320003
  9. Ty-mediated gene expression of the LYS2 and HIS4 genes of Saccharomyces cerevisiae is controlled by the same SPT genes.
    Proc Natl Acad Sci U S A. 1984 Apr;81(8):2431-4 PMID: 6326126
  10. Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae.
    Genetics. 1984 Jun;107(2):179-97 PMID: 6329902
  11. Effects of Ty insertions on HIS4 transcription in Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Jul;4(7):1246-51 PMID: 6095055
  12. Multiple specific contacts between a mammalian transcription factor and its cognate promoters.
    Nature. 1984 Nov 29-Dec 5;312(5993):409-13 PMID: 6095100
  13. The SPT3 gene is required for normal transcription of Ty elements in S. cerevisiae.
    Cell. 1984 Dec;39(3 Pt 2):675-82 PMID: 6096019
  14. A retrovirus-like strategy for expression of a fusion protein encoded by yeast transposon Ty1.
    Nature. 1985 Jan 17-23;313(5999):243-6 PMID: 2982101
  15. Ty elements transpose through an RNA intermediate.
    Cell. 1985 Mar;40(3):491-500 PMID: 2982495
  16. Nucleotide sequence of a yeast Ty element: evidence for an unusual mechanism of gene expression.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2829-33 PMID: 2581255
  17. Nucleotide sequence and characteristics of a Ty element from yeast.
    Nucleic Acids Res. 1985 Apr 25;13(8):2745-58 PMID: 2987866
  18. Variants within the yeast Ty sequence family encode a class of structurally conserved proteins.
    Nucleic Acids Res. 1985 Jun 11;13(11):4097-112 PMID: 2989787
  19. Activation regions in a yeast transposon have homology to mating type control sequences and to mammalian enhancers.
    Proc Natl Acad Sci U S A. 1985 Aug;82(16):5423-7 PMID: 2991922
  20. Transposable element sequences involved in the enhancement of yeast gene expression.
    Proc Natl Acad Sci U S A. 1985 Aug;82(16):5428-32 PMID: 2991923
  21. Functional selection and analysis of yeast centromeric DNA.
    Cell. 1985 Oct;42(3):913-21 PMID: 2996783
  22. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.
    Nature. 1986 Jan 9-15;319(6049):154-8 PMID: 3079885
  23. The relationship between the "TATA" sequence and transcription initiation sites at the HIS4 gene of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8557-61 PMID: 3909147
  24. Separation of DNA binding from the transcription-activating function of a eukaryotic regulatory protein.
    Science. 1986 Feb 14;231(4739):699-704 PMID: 3080805
  25. Identification of a telomere-binding activity from yeast.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):3713-7 PMID: 3520552
  26. GCN4 protein, a positive transcription factor in yeast, binds general control promoters at all 5' TGACTC 3' sequences.
    Proc Natl Acad Sci U S A. 1986 Nov;83(22):8516-20 PMID: 3464968
  27. The functions and relationships of Ty-VLP proteins in yeast reflect those of mammalian retroviral proteins.
    Cell. 1987 Apr 10;49(1):111-9 PMID: 3030564
  28. Yeast HAP1 activator binds to two upstream activation sites of different sequence.
    Cell. 1987 Apr 10;49(1):19-27 PMID: 3030565
  29. Contactpoint analysis of the HeLa nuclear factor I recognition site reveals symmetrical binding at one side of the DNA helix.
    EMBO J. 1987 Jan;6(1):161-8 PMID: 3034574
  30. Cell-type-dependent gene activation by yeast transposon Ty1 involves multiple regulatory determinants.
    Mol Cell Biol. 1987 Sep;7(9):3205-11 PMID: 2823122
  31. Transformation of yeast.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 PMID: 347451
  32. Contacts between Escherichia coli RNA polymerase and an early promoter of phage T7.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):122-6 PMID: 6987648
  33. The origins of gene instability in yeast.
    Science. 1980 Sep 19;209(4463):1375-80 PMID: 6251544
  34. Sequence variation in dispersed repetitive sequences in Saccharomyces cerevisiae.
    J Mol Biol. 1981 Feb 5;145(4):619-32 PMID: 7021854
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.6.2572-2580.1988
Published
1988-06-00
Pages
2572-80
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363458
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.8.6.2572-2580.1988 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2