Galactose at the nonreducing terminus of O-linked oligosaccharides of mouse egg zona pellucida glycoprotein ZP3 is essential for the glycoprotein's sperm receptor activity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 18 ·1988-09-00 ·Pages 6778-82

Bleil JD, Wassarman PM

Abstract

During fertilization in mice, zona pellucida glycoprotein ZP3 mediates initial sperm-egg interactions by serving as receptor for sperm. Purified egg ZP3, as well as ZP3-derived O-linked oligosaccharides, exhibit sperm receptor activity in vitro. We report that treatment of purified egg ZP3 and ZP3-derived O-linked oligosaccharides with either alpha-galactosidase or galactose oxidase results in loss of sperm receptor activity. In the latter case, sperm receptor activity can be restored to the oxidized glycoprotein and O-linked oligosaccharides by treatment with sodium borohydride. We conclude that galactose, located in alpha-linkage at the nonreducing terminus of O-linked oligosaccharides, is at least one of the sugar determinants on ZP3 responsible for binding of sperm to the zona pellucida.

MeSH Terms

Animals Chromatography, High Pressure Liquid Egg Proteins Female Galactose/pharmacology Galactose Oxidase/metabolism Glycoproteins/analysis Glycoside Hydrolases/metabolism Male Membrane Glycoproteins Mice Ovum/analysis Receptors, Cell Surface/metabolism Sperm-Ovum Interactions Zona Pellucida/analysis Zona Pellucida Glycoproteins

Chemicals

Egg Proteins Glycoproteins Membrane Glycoproteins Receptors, Cell Surface Zona Pellucida Glycoproteins Zp3 protein, mouse egg surface sperm receptor Galactose Oxidase Glycoside Hydrolases Galactose

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Bleil J D

Department of Cell and Developmental Biology, Roche Institute of Molecular Biology, Nutley, NJ 07110.

Wassarman P M

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23 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.85.18.6778

Published

1988-09-00

Pages

6778-82

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC282061

Subset

Corrections

ErratumIn

External Links

PubMed source DOI: 10.1073/pnas.85.18.6778 PMC Full Text View journal details

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Galactose at the nonreducing terminus of O-linked oligosaccharides of mouse egg zona pellucida glycoprotein ZP3 is essential for the glycoprotein's sperm receptor activity.

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