Glycoprotein, elastin, and collagen secretion by rat smooth muscle cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 1 ·1979-01-00 ·Pages 353-7

Jones PA, Scott-Burden T, Gevers W

Abstract

Smooth muscle cells from rat heart secreted extracellular matrix components at high rates for many generations in culture. The matrix proteins remained anchored to the culture dish and were characterized after removal of cellular material with sodium dodecyl sulfate. Sequential enzyme digestion demonstrated the presence of at least three components, including glycoprotein(s), elastin, and collagen. Prolonged extraction of the matrix with detergent under reducing conditions solubilized a fucosylated glycoprotein having an apparent molecular weight of 250,000 and two other proteins with molecular weights of 72,000 and 45,000, respectively. Sublines derived from discrete colonies of smooth muscle cells synthesized all of the matrix components, and the proportion of collagen secreted by some sublines increased with time in culture. The biosynthesis of a mixed extracellular matrix and the relationships among the component proteins were therefore studied in one system producing milligram quantities of material.

MeSH Terms

Animals Cells, Cultured Collagen/metabolism Elastin/metabolism Extracellular Space/metabolism Glycoproteins/metabolism Molecular Weight Muscle Proteins/metabolism Muscle, Smooth/metabolism Myocardium/metabolism Rats

Chemicals

Glycoproteins Muscle Proteins Collagen Elastin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Jones P A

Scott-Burden T

Gevers W

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25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.76.1.353

Published

1979-01-00

Pages

353-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC382937

Subset

External Links

PubMed source DOI: 10.1073/pnas.76.1.353 PMC Full Text View journal details

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Glycoprotein, elastin, and collagen secretion by rat smooth muscle cells.

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