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PMID: 2843814 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Physical mapping of the Mycoplasma pneumoniae genome.

Nucleic acids research ·Vol. 16 ·No. 17 ·1988-09-12 ·Pages 8323-36

Wenzel R, Herrmann R

Abstract

In order to study the genome organization of Mycoplasma pneumoniae a cosmid library of M. pneumoniae DNA was established using a newly designed cosmid vector (pcosRW2). From this library 32 overlapping clones were isolated covering a contiguous 720 kbp DNA segment representing about 90% of the genome assuming a genome size of about 800 kbp.

MeSH Terms

Base Sequence Cosmids DNA Restriction Enzymes DNA, Bacterial/genetics Genes, Bacterial Molecular Sequence Data Mycoplasma pneumoniae/genetics Nucleic Acid Hybridization Oligodeoxyribonucleotides/chemical synthesis

Chemicals

DNA, Bacterial Oligodeoxyribonucleotides DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Wenzel R

University of Heidelberg, FRG.

Herrmann R

References (17)

17 references, click to expand

Mycoplasmas (Mollicutes) have a low number of rRNA genes.
J Bacteriol. 1984 Apr;158(1):376-8 PMID: 6201476
Lambda replacement vectors carrying polylinker sequences.
J Mol Biol. 1983 Nov 15;170(4):827-42 PMID: 6315951
Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
DNA. 1985 Apr;4(2):165-70 PMID: 3996185
Total synthesis of a gene for bovine rhodopsin.
Proc Natl Acad Sci U S A. 1986 Feb;83(3):599-603 PMID: 3456156
Transposition of gram-positive transposon Tn916 in Acholeplasma laidlawii and Mycoplasma pulmonis.
Science. 1987 Mar 13;235(4794):1392-4 PMID: 3029869
Conjugal transfer of transposon Tn916 from Streptococcus faecalis to Mycoplasma hominis.
J Bacteriol. 1987 Aug;169(8):3836-9 PMID: 3038852
Oligonucleotide probes complementary to variable regions of ribosomal RNA discriminate between Mycoplasma species.
J Gen Microbiol. 1987 Jul;133(7):1969-74 PMID: 2444670
Tissue cultures and mycoplasmas.
Tex Rep Biol Med. 1965 Jun;23:Suppl 1:285+ PMID: 5833547
A complementation analysis of the restriction and modification of DNA in Escherichia coli.
J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
Genome size of mycoplasmal DNA.
Nature. 1969 Dec 20;224(5225):1209-10 PMID: 5358346
A simple method for DNA restriction site mapping.
Nucleic Acids Res. 1976 Sep;3(9):2387-98 PMID: 787937
Mycoplasmas as agents of human disease.
N Engl J Med. 1981 Jan 8;304(2):80-9 PMID: 6777700
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements.
J Mol Biol. 1983 Jun 5;166(4):477-535 PMID: 6864790
Studies on transformation of Escherichia coli with plasmids.
J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/16.17.8323

Published

1988-09-12

Pages

8323-36

Language

English

Region

England

NLM ID

0411011

PMCID

PMC338561

Subset

External Links

PubMed source DOI: 10.1093/nar/16.17.8323 PMC Full Text View journal details

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Physical mapping of the Mycoplasma pneumoniae genome.

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