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PMID: 284417 Published · ppublish English Journal Article

Chemical carcinogens produce mutations to ouabain resistance in transformable C3H/10T1/2 Cl 8 mouse fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 2 ·1979-02-00 ·Pages 930-4

Landolph JR, Heidelberger C

Abstract

Chemical carcinogens induce mutations to ouabain resistance in the transformable mouse fibroblast cell line C3H/10T1/2 Cl 8. The mutant phenotype is stable and heritable in the absence of selective agent, and dose--response curves for mutant frequency were obtained with N-menthyl-N'-nitro-N-nitrosoguanidine, 3-methylcholanthrene, benzo[a]-pyrene, N-acetoxy-N-2-acetylaminofluorene, and the anti-7,8-diol-9,10-epoxide of benzo[a]pyrene. The ratio of the malignant transformation frequency to the mutation frequency was 12 for benzo[a]pyrene and 21 for N-acetoxy-N-2-acetylaminofluorene. The development of the mutational assay reported here allows the use of this permanent cell line for comparison of mutation and transfomration frequencies and as a screening system for xenobiotics that pose mutagenic or carcinogenic hazards to mammalian cells.

MeSH Terms
2-Acetylaminofluorene/pharmacology Animals Carcinogens/pharmacology Cell Transformation, Neoplastic/drug effects Cells, Cultured Dose-Response Relationship, Drug Drug Resistance Methylnitronitrosoguanidine/pharmacology Mice Mice, Inbred C3H Mutation/drug effects Ouabain/pharmacology Time Factors
Chemicals
Carcinogens Methylnitronitrosoguanidine Ouabain 2-Acetylaminofluorene
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Landolph J R
Heidelberger C
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.2.930
Published
1979-02-00
Pages
930-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383096
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.2.930 PMC Full Text View journal details
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