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PMID: 2845137 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Translocation of rubella virus glycoprotein E1 into the endoplasmic reticulum.

Journal of virology ·Vol. 62 ·No. 11 ·1988-11-00 ·Pages 4259-64

Hobman TC, Shukin R, Gillam S

Abstract

Rubella virus (RV) contains four structural proteins, C (capsid), E2a, E2b, and E1, which are derived from posttranslational processing of a single polyprotein precursor, p110. C protein is nonglycosylated and is thought to interact with RV RNA to form a nucleocapsid. E1 and E2 are membrane glycoproteins that form the spike complexes located on the virion exterior. Two different E1 cDNAs were used to analyze the requirements for translocation of E1 into the endoplasmic reticulum. Analysis of expression of these cDNAs both in vivo and in vitro showed that RV E1 was stably expressed and glycosylated in COS cells and correctly targeted into microsomes in the absence of E2 glycoprotein. The results provide experimental evidence that translocation of RV E1 glycoprotein into the endoplasmic reticulum is mediated by a signal peptide contained within the 69 carboxyl-terminal residues of E2.

MeSH Terms
Amino Acid Sequence Base Sequence Biological Transport Blotting, Northern Cell-Free System Cells, Cultured Cloning, Molecular Endonucleases Endoplasmic Reticulum/metabolism Genes, Viral Membrane Glycoproteins/metabolism Microsomes/metabolism Molecular Sequence Data Mutation Plasmids Precipitin Tests Protein Biosynthesis Protein Sorting Signals/genetics,physiology Restriction Mapping Rubella virus/physiology Single-Strand Specific DNA and RNA Endonucleases Transfection Viral Envelope Proteins/biosynthesis,physiology Viral Proteins/metabolism Viral Structural Proteins Virus Replication
Chemicals
Membrane Glycoproteins Protein Sorting Signals Viral Envelope Proteins Viral Proteins Viral Structural Proteins Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hobman T C
Department of Pathology, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
Shukin R
Gillam S
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.11.4259-4264.1988
Published
1988-11-00
Pages
4259-64
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253859
Subset
IM
Databases
GENBANK
M22053
External Links
PubMed source DOI: 10.1128/JVI.62.11.4259-4264.1988 PMC Full Text View journal details
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