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PMID: 2846509 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of ompC and ompF expression in Escherichia coli in the absence of envZ.

Journal of bacteriology ·Vol. 170 ·No. 11 ·1988-11-00 ·Pages 5080-5

Forst S, Delgado J, Ramakrishnan G, Inouye M

Abstract

The expression of the genes encoding the major outer membrane porin proteins OmpF and OmpC in Escherichia coli is regulated by ompR, which encodes the transcriptional activator protein OmpR, and envZ, which encodes a receptorlike protein located in the inner membrane. To examine the role of EnvZ in the expression of the osmoregulated porin genes, we analyzed the production of OmpF and OmpC in cells that lack envZ function. We show that EnvZ is required for the maximal production of OmpC in cells grown in minimal medium but is not essential for the efficient induction of OmpC that occurs during a shift to a high-osmolarity medium. In contrast, the production of OmpF in cells that lack envZ function was similar to that of the parent strain, whereas OmpF repression during a shift to a high-osmolarity medium was incomplete in the absence of EnvZ. These results are discussed in the context of the putative role of EnvZ in the expression of ompF and ompC.

MeSH Terms
Bacterial Outer Membrane Proteins/biosynthesis,genetics,isolation & purification Escherichia coli/genetics Gene Expression Regulation Genes Genes, Bacterial Plasmids Porins Transcription, Genetic
Chemicals
Bacterial Outer Membrane Proteins Porins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Forst S
Department of Biochemistry, Robert Wood Johnson Medical School, Rutgers, University of Medicine and Dentistry of New Jersey, Piscataway 08854.
Delgado J
Ramakrishnan G
Inouye M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.11.5080-5085.1988
Published
1988-11-00
Pages
5080-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211574
Subset
IM
Grants
NIGMS NIH HHS · GM11553 · United States
NIGMS NIH HHS · GM19043 · United States
External Links
PubMed source DOI: 10.1128/jb.170.11.5080-5085.1988 PMC Full Text View journal details
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