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PMID: 2850466 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Posttranscriptional regulation of cytochrome c expression during the developmental cycle of Trypanosoma brucei.

Molecular and cellular biology ·Vol. 8 ·No. 11 ·1988-11-00 ·Pages 4625-33

Torri AF, Hajduk SL

Abstract

We examined the expression of a nucleus-encoded mitochondrial protein, cytochrome c, during the life cycle of Trypanosoma brucei. The bloodstream forms of T. brucei, the long slender and short stumpy trypanosomes, have inactive mitochondria with no detectable cytochrome-mediated respiration. The insect form of T. brucei, the procyclic trypanosomes, has fully functional mitochondria. Cytochrome c is spectrally undetectable in the bloodstream forms of trypanosomes, but during differentiation to the procyclic form, spectrally detected holo-cytochrome c accumulates rapidly. We have purified T. brucei cytochrome c and raised antibodies that react to both holo- and apo-cytochrome c. In addition, we isolated a partial cDNA to trypanosome cytochrome c. An examination of protein expression and steady-state mRNA levels in T. brucei indicated that bloodstream trypanosomes did not express cytochrome c but maintained significant steady-state levels of cytochrome c mRNA. The results suggest that in T. brucei, cytochrome c is developmentally regulated by a posttranscriptional mechanism which prevents either translation or accumulation of cytochrome c in the bloodstream trypanosomes.

MeSH Terms
Amino Acid Sequence Animals Apoproteins/biosynthesis Base Sequence Cytochrome c Group/biosynthesis,genetics Gene Expression Regulation Molecular Sequence Data RNA, Messenger/genetics,metabolism Transcription, Genetic Trypanosoma brucei brucei/genetics,growth & development,metabolism
Chemicals
Apoproteins Cytochrome c Group RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Torri A F
Department of Biochemistry, University of Alabama, Birmingham School of Medicine 35294.
Hajduk S L
References (44)
44 references, click to expand
  1. Antibodies against cytochromes c from vertebrates.
    J Biol Chem. 1966 Jan 10;241(1):251-3 PMID: 4158860
  2. mRNA structures influencing translation in the yeast Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 Apr;8(4):1591-601 PMID: 2837649
  3. Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides.
    Nature. 1967 Jun 24;214(5095):1302-4 PMID: 6056841
  4. Selective enzyme purification by affinity chromatography.
    Proc Natl Acad Sci U S A. 1968 Oct;61(2):636-43 PMID: 4971842
  5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  6. Biosynthesis of cytochrome c. The sites of synthesis of apoprotein and holoenzyme.
    Eur J Biochem. 1970 Feb;12(2):392-8 PMID: 5459577
  7. The amino acid sequences of cytochromes c-551 from three species of Pseudomonas.
    Biochem J. 1973 Mar;131(3):485-98 PMID: 4352718
  8. Changes in oxidative metabolism and ultrastructure accompanying differentiation of the mitochondrion in Trypanosoma brucei.
    Int J Parasitol. 1973 Sep;3(5):691-704 PMID: 4354962
  9. Change of cytochrome c structure during development of the mouse.
    Eur J Biochem. 1975 Jun 16;55(1):167-83 PMID: 240690
  10. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  11. Electron transport systems in kinetoplastida.
    Biochim Biophys Acta. 1976 Sep 27;456(2):149-93 PMID: 788794
  12. New culture medium for maintenance of tsetse tissues and growth of trypanosomatids.
    J Protozool. 1977 May;24(2):325-9 PMID: 881656
  13. Sequence of the gene for iso-1-cytochrome c in Saccharomyces cerevisiae.
    Cell. 1979 Apr;16(4):753-61 PMID: 222467
  14. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  15. A sensitive and rapid method for recombinant phage screening.
    Methods Enzymol. 1979;68:389-95 PMID: 396438
  16. Trypanosoma brucei: biochemical and morphological changes during in vitro transformation of bloodstream- to procyclic-trypomastigotes.
    Exp Parasitol. 1981 Jun;51(3):408-17 PMID: 6785103
  17. In vitro synthesis and posttranslational uptake of cytochrome c into isolated mitochondria: role of a specific addressing signal in the apocytochrome.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4368-72 PMID: 6270674
  18. The molecular biology of trypanosomes.
    Annu Rev Biochem. 1982;51:695-726 PMID: 7051964
  19. Tubulin genes are tandemly linked and clustered in the genome of trypanosoma brucei.
    Cell. 1983 Jan;32(1):35-43 PMID: 6825172
  20. Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site.
    Cell. 1983 Apr;32(4):1279-86 PMID: 6301690
  21. Elaboration of mitochondrial function during Trypanosoma brucei differentiation.
    Mol Biochem Parasitol. 1983 Jan;7(1):75-86 PMID: 6843594
  22. Receptor sites involved in posttranslational transport of apocytochrome c into mitochondria: specificity, affinity, and number of sites.
    Proc Natl Acad Sci U S A. 1983 Aug;80(16):4963-7 PMID: 6308663
  23. Protein blotting: principles and applications.
    Anal Biochem. 1983 May;131(1):1-15 PMID: 6193725
  24. Biogenesis of cytochrome c in Neurospora crassa.
    Methods Enzymol. 1983;97:261-74 PMID: 6318029
  25. Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae.
    Cell. 1984 Feb;36(2):503-11 PMID: 6319028
  26. Variant antigen genes of Trypanosoma brucei: genomic alteration of a spliced leader orphon and retention of expression-linked copies during differentiation.
    Proc Natl Acad Sci U S A. 1984 Feb;81(3):684-8 PMID: 6322164
  27. Apparent discontinuous transcription of Trypanosoma brucei variant surface antigen genes.
    Nature. 1984 Sep 27-Oct 3;311(5984):350-5 PMID: 6090933
  28. Discontinuous synthesis of mRNA in trypanosomes.
    EMBO J. 1984 Oct;3(10):2387-92 PMID: 6094183
  29. Developmental expression of nuclear genes that encode mitochondrial proteins: insect cytochromes c.
    Proc Natl Acad Sci U S A. 1985 Apr;82(7):1964-8 PMID: 2984675
  30. Developmental cycles and biology of pathogenic trypanosomes.
    Br Med Bull. 1985 Apr;41(2):105-14 PMID: 3928017
  31. Discontinuous transcription and antigenic variation in trypanosomes.
    Annu Rev Biochem. 1986;55:701-32 PMID: 2874767
  32. Transcriptional regulation of the mitochondrial genome of yeast Saccharomyces cerevisiae.
    J Biol Chem. 1986 Sep 5;261(25):11756-64 PMID: 3528145
  33. Major transcript of the frameshifted coxII gene from trypanosome mitochondria contains four nucleotides that are not encoded in the DNA.
    Cell. 1986 Sep 12;46(6):819-26 PMID: 3019552
  34. Developmental regulation of trypanosome mitochondrial gene expression.
    J Biol Chem. 1987 Jan 15;262(2):927-32 PMID: 2879836
  35. Yeast HAP1 activator binds to two upstream activation sites of different sequence.
    Cell. 1987 Apr 10;49(1):19-27 PMID: 3030565
  36. Biogenesis of mitochondria: a mutation in the 5'-untranslated region of yeast mitochondrial oli1 mRNA leading to impairment in translation of subunit 9 of the mitochondrial ATPase complex.
    Nucleic Acids Res. 1987 Mar 11;15(5):1965-77 PMID: 2951651
  37. Cytochrome c protein-synthesis rates and mRNA contents during atrophy and recovery in skeletal muscle.
    Biochem J. 1987 Jan 1;241(1):257-63 PMID: 3032156
  38. Developmentally regulated addition of nucleotides within apocytochrome b transcripts in Trypanosoma brucei.
    Cell. 1987 May 8;49(3):337-45 PMID: 3568129
  39. Transcription of kinetoplast DNA minicircles.
    Cell. 1987 Jun 5;49(5):625-32 PMID: 2438049
  40. Developmental regulation of a novel repetitive protein of Trypanosoma brucei.
    Mol Cell Biol. 1987 Aug;7(8):2838-44 PMID: 3670296
  41. Lambda gt 11: gene isolation with antibody probes and other applications.
    Methods Enzymol. 1987;154:107-28 PMID: 2448583
  42. Creation of AUG initiation codons by addition of uridines within cytochrome b transcripts of kinetoplastids.
    Proc Natl Acad Sci U S A. 1988 Jan;85(2):539-43 PMID: 2448777
  43. Developmental aspects of uridine addition within mitochondrial transcripts of Trypanosoma brucei.
    Mol Cell Biol. 1988 Mar;8(3):1259-65 PMID: 2452974
  44. Polymorphism and mitochondrial activity in sleeping sickness trypanosomes.
    Nature. 1965 Nov 20;208(5012):762-6 PMID: 5868887
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.11.4625-4633.1988
Published
1988-11-00
Pages
4625-33
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365551
Subset
IM
Grants
NIAID NIH HHS · AI-21401 · United States
Databases
GENBANK
M23360
External Links
PubMed source DOI: 10.1128/mcb.8.11.4625-4633.1988 PMC Full Text View journal details
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