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PMID: 285316 Published · ppublish English Journal Article

An endonuclease cleavage map of the plasmid pWWO-8, a derivative of the TOL plasmid of Pseudomonas putida mt-2.

Molecular & general genetics : MGG ·Vol. 168 ·No. 1 ·1979-01-05 ·Pages 97-99

Downing RG, Duggleby CJ, Villems R, Broda P

Abstract

Cleavage sites on the pWWO-8 plasmid were determined for the restriction endonucleases HindIII and XhoI. Terminal labelling using DNA polymerase I was particularly useful both for the characterisation of the smaller cleavage products and for confirmation of the order of fragments in the intact plasmid.

MeSH Terms
DNA Polymerase I/metabolism DNA Restriction Enzymes/metabolism DNA, Bacterial/metabolism Electrophoresis, Agar Gel Genes Genetic Linkage Plasmids Pseudomonas/genetics
Chemicals
DNA, Bacterial DNA Polymerase I DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Downing R G
Duggleby C J
Villems R
Broda P
References (5)
5 references, click to expand
  1. Molecular sizes and relationships of TOL plasmids in Pseudomonas.
    J Bacteriol. 1977 Jun;130(3):1274-80 PMID: 863855
  2. Restriction endonuclease mapping of DNA using in situ digestion in two-dimensional gels.
    FEBS Lett. 1978 May 15;89(2):267-70 PMID: 658415
  3. Two modes of loss of the Tol function from Pseudomonas putida mt-2.
    Mol Gen Genet. 1977 Jul 20;154(2):203-4 PMID: 895716
  4. Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5.
    J Bacteriol. 1978 Jul;135(1):227-38 PMID: 97269
  5. Nucleotide sequence analysis with polynucleotide kinase and nucleotide "mapping" methods. 5'-Terminal sequences of deoxyribonucleic acid from bacteriophages lambda and 424.
    Biochem J. 1973 Mar;131(3):569-82 PMID: 4352720
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267938
Published
1979-01-05
Pages
97-99
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267938
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