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PMID: 2861207 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Monensin-resistant mouse Balb/3T3 cell mutant with aberrant penetration of vesicular stomatitis virus.

The Journal of cell biology ·Vol. 101 ·No. 1 ·1985-07-00 ·Pages 60-5

Ono M, Mifune K, Yoshimura A, Ohnishi S, Kuwano M

Abstract

A mutant (MO-5) resistant to monensin (an ionophoric antibiotic) derived from the mouse Balb/3T3 cell line, was a poor host for vesicular stomatitis virus (VSV) or semliki forest virus (SFV) multiplication. The yield of VSV particles in MO-5 is one 100-fold reduced as is VSV-dependent RNA synthesis. In contrast to a pH-remedial mutant, the abortive production of infectious VSV particles in MO-5 cells was not restored by low pH treatment. The pH values in the endosome and the lysosome of MO-5 cells were 5.2 and 5.4, respectively, values that were comparable to the pH value in Balb/3T3 cells. Assays with [3H]uridine-labeled VSV indicated similar binding of VSV in MO-5: percoll gradient centrifugation analysis of [35S]methionine-labeled VSV-infected Balb/3T3 showed accumulation of VSV in the lysosome fraction 20 min after VSV infection, whereas VSV can be found mainly in endosome/Golgi fraction of MO-5 cells after 40 to 60 min on the percoll gradients. Degradation of [35S]methionine-labeled VSV was observed at a significant rate in Balb/3T3 cells, but not in MO-5 cells. The monensin-resistant somatic cell may thus provide a genetic route to study the mechanism of endocytosis or transport of enveloped viruses.

MeSH Terms
Animals Cell Compartmentation Cell Line Drug Resistance Endocytosis Endosomes/physiology Furans/pharmacology Hydrogen-Ion Concentration Lysosomes/physiology Mice Mice, Inbred BALB C Monensin/pharmacology Mutation RNA, Viral/biosynthesis Vesicular stomatitis Indiana virus/growth & development Viral Proteins/metabolism Virus Replication/drug effects
Chemicals
Furans RNA, Viral Viral Proteins Monensin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ono M
Mifune K
Yoshimura A
Ohnishi S
Kuwano M
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.101.1.60
Published
1985-07-00
Pages
60-5
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113637
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.101.1.60 PMC Full Text View journal details
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