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PMID: 28614383 Published · epublish English Journal Article

Regulated Erlin-dependent release of the B12 transmembrane J-protein promotes ER membrane penetration of a non-enveloped virus.

PLoS pathogens ·Vol. 13 ·No. 6 ·2017-06-00 ·Pages e1006439

Inoue T, Tsai B

Abstract

The molecular mechanism by which non-enveloped viruses penetrate biological membranes remains enigmatic. The non-enveloped polyomavirus SV40 penetrates the endoplasmic reticulum (ER) membrane to reach the cytosol and cause infection. We previously demonstrated that SV40 creates its own membrane penetration structure by mobilizing select transmembrane proteins to distinct puncta in the ER membrane called foci that likely function as the cytosol entry sites. How these ER membrane proteins reorganize into the foci is unknown. B12 is a transmembrane J-protein that mobilizes into the foci to promote cytosol entry of SV40. Here we identify two closely related ER membrane proteins Erlin1 and Erlin2 (Erlin1/2) as B12-interaction partners. Strikingly, SV40 recruits B12 to the foci by inducing release of this J-protein from Erlin1/2. Our data thus reveal how a non-enveloped virus promotes its own membrane translocation by triggering the release and recruitment of a critical transport factor to the membrane penetration site.

MeSH Terms
Cell Line Endoplasmic Reticulum/virology Gene Knockdown Techniques Humans Intracellular Membranes/metabolism,virology Membrane Proteins/metabolism Nerve Tissue Proteins/metabolism Polyomavirus Infections/metabolism Simian virus 40/physiology Virus Internalization
Chemicals
ERLIN1 protein, human ERLIN2 protein, human Membrane Proteins Nerve Tissue Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Inoue Takamasa ORCID
Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, United States of America.
Tsai Billy ORCID
Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, United States of America.
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Article Info
Journal
PLoS pathogens
Abbr.
PLoS Pathog
ISSN
1553-7374
DOI
10.1371/journal.ppat.1006439
Published
2017-06-00
Epub
2017-00-14
Pages
e1006439
Language
English
Region
United States
NLM ID
101238921
PMCID
PMC5484543
Subset
IM
Grants
NIAID NIH HHS · R01 AI064296 · United States
External Links
PubMed source DOI: 10.1371/journal.ppat.1006439 PMC Full Text View journal details
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