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PMID: 286311 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Induction of terminal differentiation in human promyelocytic leukemia cells by tumor-promoting agents.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 3 ·1979-03-00 ·Pages 1293-7

Huberman E, Callaham MF

Abstract

Human promyelocytic leukemia cells (HL-60) were induced to differentiate into mature cells by the tumor-promoting agent phorbol-12-myristate-13-acetate and other related phorbol diesters. Differentiation was determined by an increase in the percent of myelocytes, metamyelocytes, and other mature myeloid cells as well as by an increase in the percent of phagocytizing cells. Induction of differentiation could be determined after 2 days of treatment with phorbol-12-myristate-13-acetate at a dose as low as 6 X 10(11) M. A correlation was found between reported tumor-promoting activity of a series of phorbol esters and their ability to induce myeloid differentiation and to inhibit cell growth. It is suggested that tumor-promoting agents like chemicals that induce terminal differentiation in these cells, at extremely low concentrations, may be used as a tool in the study of the control of cell growth, cell differentiation, and malignancy in human leukemic cells.

MeSH Terms
Cell Differentiation/drug effects Cell Division/drug effects Cell Line Humans Kinetics Leukemia, Myeloid/physiopathology Phagocytosis Phorbol Esters/pharmacology Phorbols/pharmacology Structure-Activity Relationship
Chemicals
Phorbol Esters Phorbols
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Huberman E
Callaham M F
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.3.1293
Published
1979-03-00
Pages
1293-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383237
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.3.1293 PMC Full Text View journal details
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