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PMID: 286316 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Gene localization by chromosome fractionation: globin genes are on at least two chromosomes and three estrogen-inducible genes are on three chromosomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 3 ·1979-03-00 ·Pages 1348-52

Hughes SH, Stubblefield E, Payvar F, Engel JD, Dodgson JB, Spector D, Cordell B, Schimke RT, Varmus HE

Abstract

Chicken metaphase chromosomes were partially purified by rate zonal centrifugation, and DNA was prepared from each of the fractions of the sucrose gradient. The DNA was digested with various restriction enzymes and subjected to electrophoresis in agarose gels. The DNA was transferred to nitrocellulose filters (as described by Southern), and the filters were hybridized with cDNA probes. Four globin genes alpha A, alpha D, beta, and rho or epsilon are located on at least two chromosomes, and three of the estrogen-inducible genes of the hen oviduct--ovalbumin, ovomucoid, and transferrin--are on three different chromosomes. These experiments also confirm our earlier assignment of the endogenous viral sequence related to Rous-associated virus-0 to a separate (and larger) chromosome than the cellular sequence related to the transforming gene of avian sarcoma virus (cellular sarc), although it now appears that cellular sarc is on a small macrochromosome, rather than on a microchromosome.

MeSH Terms
Animals Base Sequence Cell Line Chickens Chromosomes/drug effects,metabolism DNA/metabolism Estrogens/pharmacology Genes Genes, Viral Globins/biosynthesis Nucleic Acid Hybridization Oviducts/drug effects,metabolism Protein Biosynthesis/drug effects Ribosomes/metabolism Transcription, Genetic/drug effects
Chemicals
Estrogens Globins DNA
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Hughes S H
Stubblefield E
Payvar F
Engel J D
Dodgson J B
Spector D
Cordell B
Schimke R T
Varmus H E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.3.1348
Published
1979-03-00
Pages
1348-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383248
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.3.1348 PMC Full Text View journal details
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