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PMID: 2866488 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

An analysis of the rate of metallothionein mRNA poly(A)-shortening using RNA blot hybridization.

Nucleic acids research ·Vol. 13 ·No. 22 ·1985-11-25 ·Pages 7929-43

Mercer JF, Wake SA

Abstract

A progressive reduction in the size of rat metallothionein-1 mRNA following induction by copper chloride or dexamethasone was demonstrated on RNA blots, and was shown to be due to shortening of the poly(A)-tail. The rate of poly(A) removal was the same in rat liver and kidney following copper chloride induction, in rat liver following dexamethasone induction, and in mouse liver following copper chloride induction. In mouse liver metallothionein-1 and 2 mRNAs were shortened at the same rate. The reduction of the poly(A) tail was more rapid in the first 5 hours (approximately 20 nucleotides/h) but much slower (approximately 3 nucleotides/h) after the poly(A)-tail had been reduced to about 60 residues. Metallothionein mRNA molecules with poly(A) tail sizes less than 30-40 nucleotides were not observed. Exonuclease digestion of the poly(A)-tail is suggested, at least in the initial rapid phase. It is hypothesized that poly(A)-tails longer than 30 are required for mRNA stability and that much longer poly(A) tails may give newly synthesized mRNA molecules a competitive advantage in protein synthesis.

MeSH Terms
Animals Copper/pharmacology Dexamethasone/pharmacology Gene Expression Regulation/drug effects Kidney/metabolism Liver/metabolism Male Metallothionein/genetics Mice Molecular Weight Nucleic Acid Hybridization Poly A/metabolism RNA Processing, Post-Transcriptional RNA, Messenger/metabolism Rats Rats, Inbred Strains
Chemicals
RNA, Messenger Poly A Copper Dexamethasone Metallothionein cuprous chloride
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mercer J F
Wake S A
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27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.22.7929
Published
1985-11-25
Pages
7929-43
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322101
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.22.7929 PMC Full Text View journal details
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